MTA3 Double Nickase Plasmid (h): sc-402739-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MTA3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MTA3 Double Nickase Plasmid (h) and MTA3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MTA3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MTA3 Antibody (428C2a): sc-81325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MTA3 Double Nickase Plasmid (h) | sc-402739-NIC | 20 µg | $410.00 | |||
MTA3 Double Nickase Plasmid (h2) | sc-402739-NIC-2 | 20 µg | $410.00 |
Human MTA3 (metastasis associated 1 family member 3) is a chromatin-associated regulator that functions as a component of the NuRD (nucleosome remodeling and deacetylase) complex, coordinating ATP-dependent remodeling with histone deacetylation to modulate transcriptional programs. By shaping enhancer and promoter accessibility, MTA3 influences processes including cell identity maintenance, proliferation, and lineage-specific differentiation, particularly in epithelial contexts. Dysregulated MTA3-dependent repression/activation networks have been linked to altered epithelial–mesenchymal transition signaling and changes in gene expression signatures observed in multiple disease-relevant models. As a nuclear epigenetic factor, MTA3 is frequently studied to connect chromatin remodeling to pathway outputs such as hormone-responsive transcription, cell cycle regulation, and stress-response programs.
MTA3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MTA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MTA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MTA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MTA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.