MT-MMP-2 Antibody (YZ-12): sc-80213

MT-MMP-2 Antibody (YZ-12) is a mouse monoclonal IgG1 antibody specifically developed to detect human membrane-type matrix metalloproteinase-2 (MT-MMP-2), targeting amino acid residues 132-165 across multiple applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). MT-MMP-2 monoclonal antibody (YZ-12) is raised against a specific epitope of activated human MT-MMP-2, ensuring robust and reliable performance in various experimental settings. Matrix metalloproteinase-2 (MT-MMP-2), also known as MT2-MMP and MMP-15, is a critical enzyme within the matrix metalloproteinase (MMP) family, predominantly localized to the cell membrane where MT-MMP-2 plays a pivotal role in degrading extracellular matrix (ECM) components, including collagen, gelatin, fibronectin, laminin, and proteoglycans. MT-MMP-2 comprises a signal peptide, propeptide domain maintaining enzyme inactivity, catalytic domain essential for proteolytic activity, hinge region, and transmembrane domain anchoring MT-MMP-2 to the cell membrane. Post-translational modifications of MT-MMP-2, such as propeptide domain cleavage by proprotein convertases, activate MT-MMP-2 to participate in ECM remodeling, cell migration, and invasion processes. Glycosylation of MT-MMP-2 influences stability, localization, and protein interactions, essential for function in tissue development, wound healing, and pathological conditions including cancer metastasis, cardiovascular diseases, and inflammatory disorders. Post-translational modifications tightly control MT-MMP-2 activation and regulation, ensuring precise modulation of enzymatic activity in response to cellular signals. Through activation of other MMPs, MT-MMP-2 drives proteolytic cascades governing ECM turnover and cellular behavior. Understanding MT-MMP-2 post-translational modifications remains crucial for elucidating disease mechanisms and developing targeted therapeutic strategies.

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MT-MMP-2 Antibody (YZ-12) References:

1. TIMP independence of matrix metalloproteinase (MMP)-2 activation by membrane type 2 (MT2)-MMP is determined by contributions of both the MT2-MMP catalytic and hemopexin C domains.  |  Morrison, CJ. and Overall, CM. 2006. J Biol Chem. 281: 26528-39. PMID: 16825197
2. Prognostic values of matrix metalloproteinase family expression in human colorectal carcinoma.  |  Asano, T., et al. 2008. J Surg Res. 146: 32-42. PMID: 17543340
3. Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library. MT-MMPs form a unique membrane-type subclass in the MMP family.  |  Takino, T., et al. 1995. J Biol Chem. 270: 23013-20. PMID: 7559440
4. cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment.  |  Will, H. and Hinzmann, B. 1995. Eur J Biochem. 231: 602-8. PMID: 7649159
5. Genes of the membrane-type matrix metalloproteinase (MT-MMP) gene family, MMP14, MMP15, and MMP16, localize to human chromosomes 14, 16, and 8, respectively.  |  Mattei, MG., et al. 1997. Genomics. 40: 168-9. PMID: 9070935
6. Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization.  |  Sato, H., et al. 1997. Genomics. 39: 412-3. PMID: 9119382
7. Membrane-type matrix metalloproteinases 1 and 2 exhibit broad-spectrum proteolytic capacities comparable to many matrix metalloproteinases.  |  d'Ortho, MP., et al. 1997. Eur J Biochem. 250: 751-7. PMID: 9461298
8. Stretch-induced membrane type matrix metalloproteinase and tissue plasminogen activator in cardiac fibroblast cells.  |  Tyagi, SC., et al. 1998. J Cell Physiol. 176: 374-82. PMID: 9648925

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MT-MMP-2 Antibody (YZ-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MT-MMP-2 Antibody (YZ-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MT-MMP-2 Antibody (YZ-12) Specifications

Alternate Product Name: MT-MMP-2

Product Clone Name: YZ-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 132-165

Epitope Target: MT-MMP-2

Epitope Species: human

Product Reactivity/Detection: MT-MMP-2 ectodomain

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and protein stabilizer

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MT-MMP-2 Antibody (YZ-12): N/A