MSH2 Antibody (25D12): sc-56163
- MSH2 Antibody (25D12) is a mouse monoclonal IgG1, cited in 5 publications, provided at 250 µl supernatant
- raised against full length MSH2 of human origin
- recommended for detection of MSH2 of human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MSH2 Antibody (25D12). This work is in progress.
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MSH2 Antibody (25D12) is a mouse monoclonal IgG1 antibody that detects MSH2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-MSH2 antibody (25D12) is available as the non-conjugated form. MSH2 (25D12) antibody recognizes a protein that plays a crucial role in the DNA mismatch repair (MMR) system, which maintains genomic stability by correcting errors during DNA replication. Deficiencies in this repair mechanism can lead to mutation accumulation, significantly increasing the risk of various cancers, including hereditary nonpolyposis colorectal cancer (HNPCC). MSH2 (25D12) monoclonal antibody targets a protein associated with microsatellite instability, a hallmark of many tumors, indicating MSH2′s vital role in hereditary cancer predisposition and broader tumorigenesis. Mutations in MSH2 and its homologs, such as MLH1, PMS1, and PMS2, in various cancers highlight MSH2′s critical function in cancer biology and potential as a therapeutic target.
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MSH2 Antibody (25D12) References:
- Conversion analysis for mutation detection in MLH1 and MSH2 in patients with colorectal cancer. | Casey, G., et al. 2005. JAMA. 293: 799-809. PMID: 15713769
- Spectrum and frequencies of mutations in MSH2 and MLH1 identified in 1,721 German families suspected of hereditary nonpolyposis colorectal cancer. | Mangold, E., et al. 2005. Int J Cancer. 116: 692-702. PMID: 15849733
- Cancer risks associated with germline mutations in MLH1, MSH2, and MSH6 genes in Lynch syndrome. | Bonadona, V., et al. 2011. JAMA. 305: 2304-10. PMID: 21642682
- Massively parallel functional testing of MSH2 missense variants conferring Lynch syndrome risk. | Jia, X., et al. 2021. Am J Hum Genet. 108: 163-175. PMID: 33357406
- Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo. | Medina-Rivera, M., et al. 2023. Nucleic Acids Res. 51: 12185-12206. PMID: 37930834
- MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. | Prolla, TA., et al. 1994. Science. 265: 1091-3. PMID: 8066446
- Mutations of two PMS homologues in hereditary nonpolyposis colon cancer. | Nicolaides, NC., et al. 1994. Nature. 371: 75-80. PMID: 8072530
- Mismatch repair and cancer. | Palombo, F., et al. 1994. Nature. 367: 417. PMID: 8107798
- Mutation of a mutL homolog in hereditary colon cancer. | Papadopoulos, N., et al. 1994. Science. 263: 1625-9. PMID: 8128251
- Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer. | Bronner, CE., et al. 1994. Nature. 368: 258-61. PMID: 8145827
- Genetic mapping of a locus predisposing to human colorectal cancer. | Peltomäki, P., et al. 1993. Science. 260: 810-2. PMID: 8484120
- Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis. | Ionov, Y., et al. 1993. Nature. 363: 558-61. PMID: 8505985
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MSH2 Antibody (25D12) | sc-56163 | 250 µl supernatant | $316.00 |