MRP5 Double Nickase Plasmid (h): sc-402443-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MRP5 Double Nickase Plasmid (h) and MRP5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCC5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP5 Antibody (E-10): sc-376965
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP5 Double Nickase Plasmid (h) | sc-402443-NIC | 20 µg | $410.00 | |||
MRP5 Double Nickase Plasmid (h2) | sc-402443-NIC-2 | 20 µg | $410.00 |
ABCC5 encodes the human ATP-binding cassette transporter MRP5, a plasma membrane efflux pump that exports organic anions including cyclic nucleotides (cGMP and cAMP) and various nucleotide analogs. By regulating intracellular cyclic nucleotide pools, MRP5 modulates second-messenger signaling with downstream effects on protein kinase activity, ion channel regulation, and transcriptional programs that influence cell proliferation and stress responses. ABCC5 activity is linked to pharmacology and toxicology through altered cellular handling of xenobiotics and antimetabolites, and dysregulation has been associated with multidrug resistance phenotypes and cancer-related signaling contexts. These properties make ABCC5/MRP5 a relevant target for investigating transporter biology, cyclic nucleotide homeostasis, and mechanisms of compound sensitivity.
MRP5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.