MRP2 Double Nickase Plasmid (h): sc-400601-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MRP2 Double Nickase Plasmid (h) and MRP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP2 Antibody (G-1): sc-518048
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP2 Double Nickase Plasmid (h) | sc-400601-NIC | 20 µg | $410.00 | |||
MRP2 Double Nickase Plasmid (h2) | sc-400601-NIC-2 | 20 µg | $410.00 |
ABCC2 encodes the human ATP-binding cassette transporter MRP2 (ABCC2), an apical efflux pump that exports glutathione, glucuronide, and sulfate conjugates, as well as a range of xenobiotics, across polarized epithelia. MRP2 supports detoxification and bile formation by coupling ATP hydrolysis to canalicular transport in hepatocytes and contributes to barrier function in intestine and kidney. Through regulation of intracellular exposure to metabolites and drugs, ABCC2 activity influences pharmacokinetics and cellular stress responses, intersecting with pathways controlling oxidative stress, conjugation metabolism, and inflammatory signaling. Genetic or functional impairment of ABCC2 is linked to conjugated hyperbilirubinemia phenotypes such as Dubin–Johnson syndrome and is widely studied for its role in drug disposition and multidrug resistance mechanisms.
MRP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.