MRP1 Double Nickase Plasmid (m): sc-421613-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MRP1 Double Nickase Plasmid (m) and MRP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Abcc1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP1 Antibody (IU2H10): sc-53130
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP1 Double Nickase Plasmid (m) | sc-421613-NIC | 20 µg | $410.00 | |||
MRP1 Double Nickase Plasmid (m2) | sc-421613-NIC-2 | 20 µg | $410.00 |
Mouse Abcc1 encodes multidrug resistance-associated protein 1 (MRP1/ABCC1), an ATP-binding cassette transporter that exports a broad range of organic anions, glutathione and glucuronide conjugates, and lipid mediators across the plasma membrane. MRP1 contributes to cellular detoxification and redox homeostasis by coupling xenobiotic efflux to glutathione-dependent metabolism, influencing intracellular signaling through regulation of leukotriene and prostaglandin transport. This transporter intersects with pathways involved in oxidative stress responses, inflammatory signaling, and barrier function, and is frequently studied in the context of multidrug resistance phenotypes in cancer models. Abcc1 also provides a functional node for investigating pharmacokinetic variability and tissue-specific transport processes in mouse systems.
MRP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Abcc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Abcc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Abcc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Abcc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.