MRP1 Double Nickase Plasmid (h): sc-400456-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MRP1 Double Nickase Plasmid (h) and MRP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP1 Antibody (QCRL-1): sc-18835
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP1 Double Nickase Plasmid (h) | sc-400456-NIC | 20 µg | $410.00 | |||
MRP1 Double Nickase Plasmid (h2) | sc-400456-NIC-2 | 20 µg | $410.00 |
ABCC1 encodes the human multidrug resistance–associated protein 1 (MRP1), an ATP-binding cassette (ABC) transporter that mediates ATP-dependent efflux of organic anions, xenobiotics, and glutathione-, glucuronide-, and sulfate-conjugated metabolites. MRP1 contributes to cellular detoxification and redox homeostasis by regulating intracellular glutathione balance and the transport of leukotriene C4 and other inflammatory mediators. Through its role in membrane transport and stress response pathways, ABCC1 activity influences intracellular drug disposition, oxidative stress handling, and barrier functions in diverse tissues. Dysregulated ABCC1/MRP1 expression or function is frequently studied in the context of multidrug resistance phenotypes, inflammatory signaling, and altered metabolite transport observed across multiple disease models.
MRP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.