MRNIP Double Nickase Plasmid (h): sc-412131-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRNIP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MRNIP Double Nickase Plasmid (h) and MRNIP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MRNIP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRNIP Antibody (H-11): sc-390012
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRNIP Double Nickase Plasmid (h) | sc-412131-NIC | 20 µg | $410.00 | |||
MRNIP Double Nickase Plasmid (h2) | sc-412131-NIC-2 | 20 µg | $410.00 |
MRNIP (MRE11-RAD50-NBS1 interacting protein) is a nuclear factor implicated in the DNA damage response through functional association with the MRN complex, which coordinates detection and processing of DNA double-strand breaks. By modulating MRN-dependent signaling and end resection dynamics, MRNIP contributes to genome stability during replication stress and influences checkpoint activation and repair pathway choice. Disruption of MRN pathway components is broadly linked to chromosomal instability phenotypes and altered sensitivity to genotoxic stressors, making MRNIP a useful node for studying DNA repair network organization. MRNIP research is relevant to mechanisms that underlie mutational accumulation, aneuploidy, and cell-cycle defects observed across diverse disease contexts.
MRNIP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MRNIP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MRNIP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MRNIP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MRNIP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.