Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MRE11 CRISPR/Cas9 KO Plasmid (h): sc-401510
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRE11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRE11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRE11 Antibody (18): sc-135992
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRE11 CRISPR/Cas9 KO Plasmid (h) | sc-401510 | 20 µg | $397.00 |
Overview
MRE11 encodes a nuclease that forms the MRN complex with RAD50 and NBN to sense DNA double-strand breaks and coordinate ATM-dependent checkpoint signaling. Through 3′–5′ exonuclease and endonuclease activities, MRE11 promotes DNA end resection that channels repair toward homologous recombination and stabilizes stalled replication forks during replication stress. This genome maintenance role links MRE11 to telomere homeostasis, meiotic recombination, and suppression of chromosomal rearrangements. Dysregulation of MRN complex function is associated with genomic instability phenotypes and has been studied in the context of cancer biology and inherited DNA repair disorders.
MRE11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MRE11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MRE11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MRE11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRE11 protein expression.
This CRISPR knockout system enables efficient generation of MRE11-deficient cell models for investigation of MRE11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MRE11 exon(s) critical for MRE11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MRE11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRE11 CRISPR/Cas9 KO Plasmid (h) and MRE11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MRE11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRE11 HDR Plasmid (h) and MRE11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MRE11 homology arms to support homology-directed repair at defined MRE11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.