MRCKβ CRISPR Activation Plasmid (h): sc-403169-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRCKβ CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- MRCKβ CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by MRCKβ CRISPR Activation Plasmid (h) and MRCKβ CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CDC42BPB transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRCKβ Antibody (C-12): sc-374597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRCKβ CRISPR Activation Plasmid (h) | sc-403169-ACT | 20 µg | $397.00 |
CDC42BPB encodes myotonic dystrophy kinase-related Cdc42-binding protein beta (MRCKβ), a serine/threonine kinase that functions downstream of Rho family GTPases, particularly CDC42, to regulate actin–myosin contractility and cytoskeletal remodeling. MRCKβ phosphorylates substrates such as myosin light chain and LIMK, influencing stress fiber formation, membrane dynamics, cell polarity, and directional migration. Through these roles, CDC42BPB connects Rho GTPase signaling to pathways controlling adhesion turnover and morphogenetic changes. Dysregulated MRCKβ activity and altered CDC42BPB expression have been associated with aberrant cell motility programs relevant to cancer invasion and metastasis models, as well as broader contexts of tissue remodeling and inflammatory microenvironments.
MRCKβ CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CDC42BPB expression without altering the underlying DNA sequence.
MRCKβ CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CDC42BPB locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CDC42BPB transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous MRCKβ expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CDC42BPB locus and enabling the study of MRCKβ-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of MRCKβ pathway restoration in tumor cells with silenced or reduced CDC42BPB expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.