Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MPO/Myeloperoxidase Double Nickase Plasmid (h): sc-400822-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MPO/Myeloperoxidase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MPO/Myeloperoxidase Double Nickase Plasmid (h) and MPO/Myeloperoxidase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MPO. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MPO light chain/Myeloperoxidase Antibody (A-5): sc-365436
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MPO/Myeloperoxidase Double Nickase Plasmid (h) | sc-400822-NIC | 20 µg | $410.00 | |||
MPO/Myeloperoxidase Double Nickase Plasmid (h2) | sc-400822-NIC-2 | 20 µg | $410.00 |
MPO encodes myeloperoxidase, a heme-containing peroxidase stored in neutrophil azurophilic granules and released during degranulation and neutrophil extracellular trap (NET) formation. MPO catalyzes the conversion of hydrogen peroxide and chloride into hypochlorous acid and related reactive oxidants, linking the NADPH oxidase–dependent respiratory burst to antimicrobial effector chemistry and redox signaling. Through oxidation of lipids, proteins, and nucleic acids, MPO activity influences inflammatory signaling cascades, endothelial dysfunction, and immune cell recruitment. Altered MPO expression or activity has been associated with dysregulated innate immunity and oxidative stress phenotypes relevant to inflammatory and vascular disease biology, as well as tumor microenvironment studies.
MPO/Myeloperoxidase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MPO locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MPO. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MPO function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MPO-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.