MPG1 Double Nickase Plasmid (h2): sc-409072-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MPG1 Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MPG1 Double Nickase Plasmid (h2) and MPG1 Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting MPEG1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MPG1 Double Nickase Plasmid (h2) | sc-409072-NIC-2 | 20 µg | $410.00 |
Human MPEG1 encodes macrophage-expressed gene 1 (MPG1), a pore-forming protein implicated in innate immune defense and antimicrobial activity within phagolysosomal compartments. MPG1 participates in myeloid cell effector programs that couple pathogen recognition to vesicular trafficking, membrane disruption, and inflammatory signaling, linking it to processes such as phagocytosis, lysosome function, and cytokine-regulated activation states. Altered MPEG1 expression has been reported in contexts of infection, chronic inflammation, and tumor-associated macrophage biology, supporting investigation of its role in immune cell polarization and tissue microenvironment remodeling. Gene editing of MPEG1 enables mechanistic studies in primary macrophages or immune cell models to dissect host–pathogen interactions, intracellular killing pathways, and immune dysregulation phenotypes relevant to inflammatory and cancer research.
MPG1 Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the MPEG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MPEG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MPEG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MPEG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.