Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MOZ Double Nickase Plasmid (h): sc-403564-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MOZ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MOZ Double Nickase Plasmid (h) and MOZ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KAT6A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MOZ Antibody (4D8): sc-293283
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOZ Double Nickase Plasmid (h) | sc-403564-NIC | 20 µg | $410.00 | |||
MOZ Double Nickase Plasmid (h2) | sc-403564-NIC-2 | 20 µg | $410.00 |
KAT6A (MOZ) encodes a MYST family histone acetyltransferase that primarily acetylates histone H3 (including H3K9 and H3K14) to promote open chromatin and transcriptional activation. MOZ functions in multiprotein chromatin-regulatory complexes and coordinates enhancer and promoter activity that influences cell-cycle progression, DNA damage responses, and lineage-specific gene expression programs. In hematopoietic and developmental contexts, KAT6A helps maintain transcriptional states required for progenitor self-renewal and differentiation. Dysregulation of KAT6A through mutation, altered dosage, or chromosomal rearrangements has been linked to aberrant transcriptional control and oncogenic or neurodevelopmental phenotypes, making it a useful node for epigenetics-focused mechanism studies.
MOZ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KAT6A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KAT6A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KAT6A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KAT6A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.