m-IgG2b BP-CFL 647: sc-542748
- mouse IgG2b binding protein (m-IgG2b BP) conjugated to CruzFluor™ 647 (CFL 647)
- supplied at 50 µg in 0.5 ml volume
- CruzFluor™ 647 (CFL 647) conjugated secondaries are similar to Alexa Fluor® 647 and Cy5® fluorescent dyes, offering comparable photostability and the ability to resist protein quenching
- Excitation of 654 nm, Emission of 669 nm
- Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
- Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
- Suitable for binding to most, but not all, mouse IgG2b immunoglobulins; not suitable for use with mouse monoclonal IgG1, IgG2a, IgG3, IgM, IgA or IgE antibodies
- Not cross reactive with human, rat or goat IgG antibodies
- Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
- Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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Mouse IgG2b binding protein (IgG2b BP) conjugated to CruzFluor™ 647 (CFL 647) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for RGB Western Blotting (WB), immunofluorescence (IF) and flow cytometry (FCM) signal enhancement. CruzFluor™ 647 (CFL 647) is a far-red fluorescent dye that is an excellent substitute for AlexaFluor® 647, offering comparable photostability and the ability to resist protein quenching. Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems. Mouse IgG2b binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgG2b binding protein (m-IgG2b BP) is suitable for for binding to most, but not all mouse monoclonal IgG2b antibodies; not suitable for use with mouse monoclonal IgG1, IgG2a, IgG3, IgM, IgA or IgE antibodies. Not cross reactive with human, rat or goat IgG antibodies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
m-IgG2b BP-CFL 647 | sc-542748 | 50 µg/0.5 ml | $93.00 |