Date published: 2025-10-20

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m-IgGκ BP-CFL 647: sc-516179

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Datasheets
  • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 647 (CFL 647)
  • CruzFluor™ 647 (CFL 647) conjugated secondaries are similar to Alexa Fluor® 647 and Cy5® fluorescent dyes, offering comparable photostability and the ability to resist protein quenching
  • Excitation of 654 nm, Emission of 669 nm
  • supplied at 200 µg in 0.5 ml volume
  • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
  • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
  • Suitable for binding to most, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.

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Mouse IgGκ light chain binding protein (m-IgGκ BP) conjugated to CruzFluor™ 647 (CFL 647) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB), Immunofluorescence (IF) and Flow Cytometry (FCM) signal enhancement. Mouse IgGκ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGκ light chain binding protein (m-IgGκ BP) is suitable for binding to mouse IgGκ light chain immunoglobulins, comprising a vast majority of mouse monoclonal primary antibodies; not suitable for use with mouse monoclonal IgGλ light chain primary antibodies. CruzFluor™ 647 is a far-red fluorescent dye that is an excellent substitute for AlexFluor® 647 or Cy5®, offering comparable photostability and the ability to resist protein quenching. Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

Cy5® is a trademark of GE Healthcare

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgGκ BP-CFL 647

sc-516179
200 µg/0.5 ml
$92.00