Date published: 2025-10-20

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m-IgGκ BP-CFL 555: sc-516177

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Datasheets
  • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 555 (CFL 555)
  • supplied at 200 µg in 0.5 ml volume
  • CruzFluor™ 555 (CFL 555) is a orange/red fluorescent dye that is an excellent substitute for Alexa Fluor® 555, offering comparable photostability and the ability to resist protein quenching
  • Excitation of 556 nm, Emission of 569 nm
  • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
  • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
  • Suitable for binding to most, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.

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Mouse IgGκ light chain binding protein (m-IgGκ BP) conjugated to CruzFluor™ 555 (CFL 555) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB), Immunofluorescence (IF) and Flow Cytometry (FCM) signal enhancement. Mouse IgGκ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGκ light chain binding protein (m-IgGκ BP) is suitable for binding to mouse IgGκ light chain immunoglobulins, comprising a vast majority of mouse monoclonal primary antibodies; not suitable for use with mouse monoclonal IgGλ light chain primary antibodies. CruzFluor™ 555 is an orange fluorescent dye that is an excellent substitute for AlexFluor® 555, offering comparable photostability and the ability to resist protein quenching. Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems..

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgGκ BP-CFL 555

sc-516177
200 µg/0.5 ml
$92.00

For the secondary biding to Mouse IgG1 kappa, which binding protein is better, m-IgGκ BP-CFL 555: sc-516177 or m-IgG1 BP-CFL 555: sc-533662?

Asked by: Sebastain
Thank you for your question. For assistance with a recommended secondary please contact technical service . Please call (800)-4587-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2024-11-05

I am looking for a suitable secondary antibody or binding protein for Mouse IgG1 kappa on mouse brain tissue. Does m-IgGκ BP-CFL 555 (sc-516177) produce minimal background on mouse brain tissue?

Asked by: Sebastain
Thank you for your question. There should be little to no backgorund with using mouse samples . Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
Answered by: BlakeJ
Date published: 2024-11-05

What is it for?

Asked by: O man
Thank you for your question. This product is a mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 555 (CFL 555). This mouse IgG binding protein is a strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for use in immunofluorescence (IF) and Flow Cytometry (FCM) research applications. It is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. CruzFluor™ 555 (CFL 555) is a orange/red fluorescent dye that is an excellent substitute for Alexa Fluor® 555, but CruzFluor™ dyes have a superior ability to resist protein quenching, and offer greater photostability. Please contact our Technical Service department if you would like further information about our new mouse IgG binding proteins. Please call us directly at (800) 457-3801 option 2, email scbt@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-06
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Rated 5 out of 5 by from Works great for Western BlotThis detection reagent worked well for detecting β-Actin (C4) antibody, sc-47778, in a fluorescent western blot assay. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Satisfactory resultsImmunofluorescence is very clear,according to the recommended dilution concentration
Date published: 2022-09-15
Rated 5 out of 5 by from 东西不错这产品适合我现在的需要 需要购买一些来使用,希望实验做下来可以顺利。期待后期可以继续使用贵公司的产品
Date published: 2018-05-14
Rated 5 out of 5 by from Good results in WBI was working with fluorescent WB and this 2ry ab worked perfectly!
Date published: 2018-02-01
Rated 5 out of 5 by from BrilliantExcellent on HEK293 and cryosections of chick embryos
Date published: 2018-01-03
Rated 5 out of 5 by from Works greatUse this secondary ab for IF of cell samples, works great.
Date published: 2017-12-05
Rated 5 out of 5 by from GreatThis sample shows highly specific staining and great signal in HeLa cells.
Date published: 2017-11-27
Rated 5 out of 5 by from GREATI use this antibody (1:100) in my Leukemia cells, it works very well.
Date published: 2017-11-20
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m-IgGκ BP-CFL 555 is rated 5.0 out of 5 by 18.
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