Date published: 2026-11-9

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mouse anti-rabbit IgG-HRP: sc-2357

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Datasheets
  • mouse monoclonal secondary antibody conjugated to Horseradish Peroxidase (HRP)
  • supplied at 200 µg in 0.5 ml volume
  • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Enhanced Chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
  • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
  • Also see our mouse anti-rabbit IgG-FITC, PE, PE-Cy7, TRITC, Texas Red®, PerCP, PerCP-Cy5.5, CFL 488, CFL 555, CFL 594, CFL 647, CFL 680, and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-rabbit secondary antibody fluorescent dye conjugates.
  • For a Cruz Marker™ compatible anti-rabbit monoclonal secondary antibody, use mouse anti-rabbit IgG-HRP (Cruz Marker) (sc-2357-CM) (Click here for datasheet)
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Santa Cruz Biotechnology's secondary antibodies are available conjugated to either an enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, immunostaining, flow cytometry and ELISA. Secondary antibodies are commonly affinity purified against immobilized whole IgG isotypes, including IgG1, IgG2a, IgG2b, IgG3 and IgG4. Santa Cruz Biotechnology offers a wide selection of secondary antibodies, which are used in conjunction with our Cruz Marker™ molecular weight standards. We also provide specialized secondaries, such as pre-adsorbed secondary antibodies, which are pre-adsorbed with human IgG and mouse IgG for immunoglobulin-rich tissues and cells, F(ab')2 fragment secondary antibodies that reduce non-specific secondary antibody binding to Fc receptors on the cell surface, and isotype-specific secondary antibodies against IgM, IgA and IgY.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

mouse anti-rabbit IgG-HRP

sc-2357
200 µg/0.5 ml
$78.00

mouse anti-rabbit IgG-HRP (Cruz Marker)

sc-2357-CM
200 µg/0.5 ml
$78.00

Can I get the CoA??? Cat# sc-2357, Lot# K0718 Cat# sc-47778, Lot# G3019 Cat# sc-516102, Lot# H2819

Asked by: wls11
Thank you for your question. We don't provide CoA for antibodies.
Answered by: Technical Service SH
Date published: 2026-11-04

Hello, does this antibody recognize the CH2 or CH3 of a rabbit antibody? Thank you.

Asked by: Tarla
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-10

Hi, I was wondering if this antibody detected denatured IgG, which may interfere the detection of target protein in IP experiment.

Asked by: zzzzh
Thank you for your question. It is a secondary antibody, which can work with the primary antibody, the primary antibody should be the rabbit IgG. 感谢提问。 这是二抗,需要与兔来源的IgG类型的一抗工作。
Answered by: Ling Chen
Date published: 2024-05-10

Hi, is this antibody tested for cross-reactivity with human IgG? I need a secondary anti-rabbit antibody which is not reactive with human IgG.

Asked by: damiana
Thank you for your question. We do not have any data that would indicate any kind of cross-reactivity of this secondary antibody with human Ig.
Answered by: Tech Support Europe
Date published: 2022-04-21

Is this secondary antibody (mouse anti-rabbit IgG-HRP: sc-2357) suitable for primary antibody Anticaspase-3 Antibody (31A1067): sc-56053?

Asked by: 555inr
Thank you for your question. caspase-3 Antibody (31A1067) is a mouse monoclonal IgG1 and cannot be detected with mouse anti-rabbit IgG-HRP: sc-2357. Please refer to our anti-mouse binding proteins for secondary detection reagents.
Answered by: Tech Support Europe
Date published: 2021-04-14

Hi Can I use this to detect rabbit monoclonal antibodies I am using for IHC on mouse/ rat brain sections?

Asked by: Dubey
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-02-02

Hello, Can I use these antibodies for ELISA, and what dilution do you recommend to start with ? Thank you for your advice Kind regards Oleg

Asked by: Oleg
Thank you for your question. Yes, this can be used for ELISA. We do not have a specific protocol for using HRP conjugated secondary antibodies for ELISA. The optimal secondary antibody concentration should be determined by titration.
Answered by: Technical Support
Date published: 2022-09-28

Is this natibody working in mouse brain homogenates?

Asked by: Sarah77
Thank you very much for your question. For mouse species we highly recommend m-IgGκ binding protein sc-516102. For the suitable conjugate please visit < https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4 > or contact our Technical Service by phone, email or by Live Chat
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21
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Rated 5 out of 5 by Western Blot analysis from Detection of rabbit proteins with chemiluminesceThis antibody played a key role in detecting various rabbit antibodies, providing an easily detectable band via chemiluminescence. Its compatibility with different rabbit antibodies enhanced the versatility of our experiments, making it a valuable tool in our antibody repertoire.
Date published: 2024-01-04
Rated 4 out of 5 by Bhava from Secondary antibodySecondary rabbit antibody 1/2000 dilution, works well in western blotting.
Date published: 2023-06-30
Rated 5 out of 5 by Linda3 from Good AbThe antibody is good and works fine. There are no problems with it.
Date published: 2023-02-22
Rated 5 out of 5 by a20220522 from 功能很强大的抗体已用了多次,很喜欢。效果很好,大家1:15000稀释都可以做出来。背景低,重复率高,使用 多次都没有 问题,超过很多公司的产品。
Date published: 2022-05-22
Rated 5 out of 5 by zmm2020 from very good! excelent! 非常好用。很早就用了,效果一直很稳定。重复性和稳定性也很好,很值得推荐,背景很弱,很多研究生都能完好的重复出理想的结果。
Date published: 2021-10-17
Rated 5 out of 5 by gabs01 from Good secondary antibody for western blotworked well for detection of a variety of primary antibodies in rat placenta samples.
Date published: 2021-08-12
Rated 4 out of 5 by Stevie from mouse anti-rabbit IgG-HRPworks well for western blotting of human cancer cell lines
Date published: 2021-02-13
Rated 4 out of 5 by t888 from great secondary antibodyclean and good secondary antibody. it is clean in western blot
Date published: 2020-12-01
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mouse anti-rabbit IgG-HRP Secondary Antibody is rated 4.5 out of 5 by 54.
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