mouse anti-rabbit IgG-HRP mouse monoclonal secondary antibody conjugated to horseradish peroxidase (HRP), for WB, IHC(P) and ELISA

mouse anti-rabbit IgG-HRP: sc-2357

mouse anti-rabbit IgG-HRP Secondary Antibody is rated 4.4 out of 5 by 45.
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Datasheets

    • mouse monoclonal secondary antibody conjugated to Horseradish Peroxidase (HRP)
    • supplied at 200 µg in 0.5 ml volume
    • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Enhanced Chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
    • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
    • For a Cruz Marker™ compatible anti-rabbit monoclonal secondary antibody, use mouse anti-rabbit IgG-HRP (Cruz Marker) (sc-2357-CM) (Click here for datasheet)
    • If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-HRP (sc-2357) or mouse anti-rabbit IgG-HRP (Cruz Marker) (sc-2357-CM), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-HRP secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.
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Hello, Can I use these antibodies for ELISA, and what dilution do you recommend to start with ? Thank you for your advice Kind regards Oleg

Asked by: Oleg
Thank you for your question. Yes, this can be used for ELISA. We do not have a specific protocol for using HRP conjugated secondary antibodies for ELISA. The optimal secondary antibody concentration should be determined by titration.
Answered by: Technical Support
Date published: 2019-04-20

Is this natibody working in mouse brain homogenates?

Asked by: Sarah77
Thank you very much for your question. For mouse species we highly recommend m-IgGκ binding protein sc-516102. For the suitable conjugate please visit < https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4 > or contact our Technical Service by phone, email or by Live Chat
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Hi,  I was wondering if I could get a quote on this item and shipment to Aus.  Cheers,  Melanie. 

Asked by: MelanieRoseWalker
Thank you for the question. A colleague from the Customer Service Dept. will contact you today and provide requested product quotation.
Answered by: Technical Support Europe
Date published: 2018-01-09

I need anti-rabbit IgG-HRP and anti-mouse IgG-HRP, which are light-chain specific. These secondary antibodies should not bind to heavy chain of CoIP samples.

Asked by: Uroos
Thank you for your question. We do not have an anti-rabbit antibody that recognizes specifically the light chain, but I would recommend using the mouse IgG binding protein for the mouse monoclonal antibody. You can find it here: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp Please contact us if you have any further questions or if you would like to try a sample of the binding protein.
Answered by: Tech Service
Date published: 2017-06-13
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Rated 5 out of 5 by from great secondary antibody We are using this antibody daily in our lab, and we never had any problem with it.
Date published: 2019-07-04
Rated 5 out of 5 by from Easy to use for IHC I used this antibody for immunohistochemistry assay, and I obtained clear and reliable results in human tissue (dilution 1:200)
Date published: 2018-11-05
Rated 5 out of 5 by from buen resultado buenos resultados, funciona bien, no hay problemas de especificidad
Date published: 2018-09-07
Rated 4 out of 5 by from Good signal This antibody works well on human and mouse samples in WB
Date published: 2018-08-01
Rated 5 out of 5 by from works perfectly for IHC We use this secondary antibody for IHC (dilution 1:100), always worked.
Date published: 2018-07-23
Rated 4 out of 5 by from Good antibodies I used this antibodies for WB in dilution 1:5000. It works good.
Date published: 2018-06-10
Rated 5 out of 5 by from Second AB I bought this one two months ago. It works very well, low background
Date published: 2018-04-27
Rated 4 out of 5 by from Good antibodies I get a trial package from the company. I used this secondary antibodies for WB with dilution 1:5000. It works good.
Date published: 2018-04-04
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