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mouse anti-rabbit IgG-HRP Secondary Antibody | SCBT - Santa Cruz Biotechnology
mouse anti-rabbit IgG-HRP mouse monoclonal secondary antibody conjugated to horseradish peroxidase (HRP), for WB, IHC(P) and ELISA

mouse anti-rabbit IgG-HRP: sc-2357

mouse anti-rabbit IgG-HRP Secondary Antibody is rated 4.5 out of 5 by 51.
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Datasheets

    • mouse monoclonal secondary antibody conjugated to Horseradish Peroxidase (HRP)
    • supplied at 200 µg in 0.5 ml volume
    • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Enhanced Chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
    • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
    • Also see our mouse anti-rabbit IgG-FITC, PE, PE-Cy7, TRITC, Texas Red®, PerCP, PerCP-Cy5.5, CFL 488, CFL 555, CFL 594, CFL 647, CFL 680, and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-rabbit secondary antibody fluorescent dye conjugates.
    • For a Cruz Marker™ compatible anti-rabbit monoclonal secondary antibody, use mouse anti-rabbit IgG-HRP (Cruz Marker) (sc-2357-CM) (Click here for datasheet)
    • If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-HRP (sc-2357) or mouse anti-rabbit IgG-HRP (Cruz Marker) (sc-2357-CM), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-HRP secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.
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Can I get the CoA??? Cat# sc-2357, Lot# K0718 Cat# sc-47778, Lot# G3019 Cat# sc-516102, Lot# H2819

Asked by: wls11
Thank you for your question. We don't provide CoA for antibodies.
Answered by: Technical Service SH
Date published: 2023-01-04

Hello, does this antibody recognize the CH2 or CH3 of a rabbit antibody? Thank you.

Asked by: Tarla
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Anonymous
Date published: 2022-08-10

Hi, I was wondering if this antibody detected denatured IgG, which may interfere the detection of target protein in IP experiment.

Asked by: zzzzh
Thank you for your question. It is a secondary antibody, which can work with the primary antibody, the primary antibody should be the rabbit IgG. 感谢提问。 这是二抗,需要与兔来源的IgG类型的一抗工作。
Answered by: Ling Chen
Date published: 2022-07-07

Hi, is this antibody tested for cross-reactivity with human IgG? I need a secondary anti-rabbit antibody which is not reactive with human IgG.

Asked by: damiana
Thank you for your question. We do not have any data that would indicate any kind of cross-reactivity of this secondary antibody with human Ig.
Answered by: Tech Support Europe
Date published: 2022-04-21

Is this secondary antibody (mouse anti-rabbit IgG-HRP: sc-2357) suitable for primary antibody Anticaspase-3 Antibody (31A1067): sc-56053?

Asked by: 555inr
Thank you for your question. caspase-3 Antibody (31A1067) is a mouse monoclonal IgG1 and cannot be detected with mouse anti-rabbit IgG-HRP: sc-2357. Please refer to our anti-mouse binding proteins for secondary detection reagents.
Answered by: Tech Support Europe
Date published: 2021-04-14

Hi Can I use this to detect rabbit monoclonal antibodies I am using for IHC on mouse/ rat brain sections?

Asked by: Dubey
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-02-02

Hello, Can I use these antibodies for ELISA, and what dilution do you recommend to start with ? Thank you for your advice Kind regards Oleg

Asked by: Oleg
Thank you for your question. Yes, this can be used for ELISA. We do not have a specific protocol for using HRP conjugated secondary antibodies for ELISA. The optimal secondary antibody concentration should be determined by titration.
Answered by: Technical Support
Date published: 2022-09-28

Is this natibody working in mouse brain homogenates?

Asked by: Sarah77
Thank you very much for your question. For mouse species we highly recommend m-IgGκ binding protein sc-516102. For the suitable conjugate please visit < https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4 > or contact our Technical Service by phone, email or by Live Chat
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21
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Rated 5 out of 5 by from 功能很强大的抗体 已用了多次,很喜欢。效果很好,大家1:15000稀释都可以做出来。背景低,重复率高,使用 多次都没有 问题,超过很多公司的产品。
Date published: 2022-05-22
Rated 5 out of 5 by from very good! excelent! 非常好用。 很早就用了,效果一直很稳定。重复性和稳定性也很好,很值得推荐,背景很弱,很多研究生都能完好的重复出理想的结果。
Date published: 2021-10-17
Rated 5 out of 5 by from Good secondary antibody for western blot worked well for detection of a variety of primary antibodies in rat placenta samples.
Date published: 2021-08-12
Rated 4 out of 5 by from mouse anti-rabbit IgG-HRP works well for western blotting of human cancer cell lines
Date published: 2021-02-13
Rated 4 out of 5 by from great secondary antibody clean and good secondary antibody. it is clean in western blot
Date published: 2020-12-01
Rated 5 out of 5 by from protein bands showed in a TMB chromogenic assay I bought this 2nd antibody about two weeks ago. I got the GAPDH and Catenin bands in the chromogenic assay using TEM substrate in my first running. So, mouse anti-rabbit IgG-HRP from SANTA CRUZ is a good 2nd antibody!
Date published: 2020-07-16
Rated 5 out of 5 by from great secondary antibody We are using this antibody daily in our lab, and we never had any problem with it.
Date published: 2019-07-04
Rated 5 out of 5 by from Easy to use for IHC I used this antibody for immunohistochemistry assay, and I obtained clear and reliable results in human tissue (dilution 1:200)
Date published: 2018-11-05
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