Date published: 2025-12-17

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mouse anti-rabbit IgG-FITC: sc-2359

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Datasheets
  • mouse monoclonal secondary antibody conjugated to fluorescein (FITC)
  • supplied at 200 µg in 0.5 ml volume
  • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
  • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
  • While we continue to offer conventional fluorescein (FITC) conjugates (525 nm emission) for immunofluorescent cell staining, we strongly recommend CruzFluor™ 488 conjugated Secondary antibodies (525 nm emission) as alternatives and much superior reagents.

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Santa Cruz Biotechnology's high quality, well characterized monoclonal secondary antibodies are available conjugated to either an enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, immunostaining and flow cytometry. Santa Cruz secondary antibodies are commonly affinity purified against immobilized whole IgG isotypes, including IgG1, IgG2a, IgG2b, IgG3 and IgG4. Monoclonal secondary antibodies are available conjugated to:
HRP for Western Blotting (WB) and Immunohistochemistry (IHC),
(CM) or Cruz Marker form of HRP conjugated secondary antibodies are suitable for use with our Cruz Marker™ molecular weight standards
FITC (fluorescein isothiocyanate) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
PE (phycoerythrin) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
B (Biotin) for Immunohistochemistry (IHC)
CruzFluor™ 488, 555, 594 and 647 for RGB Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
CruzFluor™ 680 and 790 for Near-Infrared (NIR) Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
AP (alkaline phosphatase) for Western Blotting (WB)
R (TRITC: tetramethyl rhodamine iso-thiocyanate) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunohistochemistry (IHC)
TR (Texas Red®) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunohistochemistry (IHC)
PerCP (peridinin chlorophyll protein complex) and PerCP-Cy5.5 (peridinin chlorophyll protein complex with cyanin-5.5) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

mouse anti-rabbit IgG-FITC

sc-2359
200 µg/0.5 ml
$77.00

Dear SCBT team, I would like to ask whether the antibodies sc-2359 and sc-516253 are the same clone, to which part of rabbit IgG it binds and which mouse isotype this antibodies are. Thank you and best regards, Valentyna

Asked by: Valentyna
Thank you for your question. Yes, these mouse anti-rabbit secondary antibodies are the same clone. They have been isotyped as IgG1, kappa light chain. The region of binding has not been characterized, but clone only binds intact non-denatured rabbit IgG and will not bind denatured rabbit heavy and light chains. Please contact our Technical Service Department by phone, e-mail or live chat if you have any further questions.
Answered by: Tech Service
Date published: 2017-10-19

Hi. Could you please confirm if this item is in stock?

Asked by: Oleksandr
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Rated 5 out of 5 by from AwesomeI bought many antibodies from santa cruz in the past and they worked really good
Date published: 2019-11-05
Rated 5 out of 5 by from Works GreatThis antibody works very good, recommended strongly~~
Date published: 2018-01-31
Rated 5 out of 5 by from Great antibody for immunochemI also test this antibody with liver tissue and it works good. 1:250
Date published: 2017-10-29
Rated 4 out of 5 by from good antibody for IF experimentI have tested this antibody for IF experiment and got a good result.
Date published: 2017-10-05
Rated 5 out of 5 by from Works well for flow cytometryWorks well for flow cytometry in mouse cells (dendritic cells)
Date published: 2017-07-11
Rated 5 out of 5 by from great antibodyfor immunohistochemistry, 1:500 worked well using tissue extracted from the brain.
Date published: 2017-07-04
Rated 2 out of 5 by from Buen resultadoEl producto fue comprado por un laboratorio con el que trabajo en colaboración y el resultado fue aceptable. Se requerirían más pruebas para ganar mejores imágenes, debido a que como fue usado con un anticuerpo primario policlonal, la intensidad de la respuesta es más variable.
Date published: 2017-06-19
Rated 5 out of 5 by from This antibody works well in flow cytometryThis antibody works well in flow cytometry and no complaints.
Date published: 2015-10-08
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mouse anti-rabbit IgG-FITC is rated 4.5 out of 5 by 8.
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