mouse anti-rabbit IgG-FITC mouse monoclonal secondary antibody conjugated to fluorescein (FITC), for WB, IF, IHC(P) and FCM

mouse anti-rabbit IgG-FITC: sc-2359

mouse anti-rabbit IgG-FITC Secondary Antibody is rated 4.5 out of 5 by 8.
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Datasheets

    • mouse monoclonal secondary antibody conjugated to fluorescein (FITC)
    • supplied at 200 µg in 0.5 ml volume
    • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
    • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
    • While we continue to offer conventional fluorescein (FITC) conjugates (525 nm emission) for immunofluorescent cell staining, we strongly recommend CruzFluor™ 488 conjugated Secondary antibodies (525 nm emission) as alternatives and much superior reagents.
    • If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-FITC (sc-2359), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-FITC secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.
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Dear SCBT team, I would like to ask whether the antibodies sc-2359 and sc-516253 are the same clone, to which part of rabbit IgG it binds and which mouse isotype this antibodies are. Thank you and best regards, Valentyna

Asked by: Valentyna
Thank you for your question. Yes, these mouse anti-rabbit secondary antibodies are the same clone. They have been isotyped as IgG1, kappa light chain. The region of binding has not been characterized, but clone only binds intact non-denatured rabbit IgG and will not bind denatured rabbit heavy and light chains. Please contact our Technical Service Department by phone, e-mail or live chat if you have any further questions.
Answered by: Tech Service
Date published: 2017-10-19
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Rated 5 out of 5 by from Awesome I bought many antibodies from santa cruz in the past and they worked really good
Date published: 2019-11-05
Rated 5 out of 5 by from Works Great This antibody works very good, recommended strongly~~
Date published: 2018-01-31
Rated 5 out of 5 by from Great antibody for immunochem I also test this antibody with liver tissue and it works good. 1:250
Date published: 2017-10-29
Rated 4 out of 5 by from good antibody for IF experiment I have tested this antibody for IF experiment and got a good result.
Date published: 2017-10-05
Rated 5 out of 5 by from Works well for flow cytometry Works well for flow cytometry in mouse cells (dendritic cells)
Date published: 2017-07-11
Rated 5 out of 5 by from great antibody for immunohistochemistry, 1:500 worked well using tissue extracted from the brain.
Date published: 2017-07-04
Rated 2 out of 5 by from Buen resultado El producto fue comprado por un laboratorio con el que trabajo en colaboración y el resultado fue aceptable. Se requerirían más pruebas para ganar mejores imágenes, debido a que como fue usado con un anticuerpo primario policlonal, la intensidad de la respuesta es más variable.
Date published: 2017-06-19
Rated 5 out of 5 by from This antibody works well in flow cytometry This antibody works well in flow cytometry and no complaints.
Date published: 2015-10-08
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