Date published: 2025-12-18

1-800-457-3801

SCBT Portrait Logo
Seach Input

mouse anti-rabbit IgG-CFL 790: sc-516253

5.0(1)
Write a reviewAsk a question

Datasheets
  • mouse monoclonal secondary antibody conjugated to CruzFluor™ 790 (CFL 790)
  • supplied at 200 µg in 0.5 ml volume
  • CruzFluor™ 790 (CFL 790) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 790, offering comparable photostability and the ability to resist protein quenching
  • Excitation of 786 nm, Emission of 811 nm
  • Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
  • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
  • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
  • For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary and secondary antibody incubations.

QUICK LINKS

Santa Cruz Biotechnology's high quality, well characterized monoclonal secondary antibodies are available conjugated to either an enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, immunostaining and flow cytometry. Santa Cruz secondary antibodies are commonly affinity purified against immobilized whole IgG isotypes, including IgG1, IgG2a, IgG2b, IgG3 and IgG4. Monoclonal secondary antibodies are available conjugated to:
HRP for Western Blotting (WB) and Immunohistochemistry (IHC),
(CM) or Cruz Marker form of HRP conjugated secondary antibodies are suitable for use with our Cruz Marker™ molecular weight standards
FITC (fluorescein isothiocyanate) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
PE (phycoerythrin) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
B (Biotin) for Immunohistochemistry (IHC)
CruzFluor™ 488, 555, 594 and 647 for RGB Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
CruzFluor™ 680 and 790 for Near-Infrared (NIR) Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
AP (alkaline phosphatase) for Western Blotting (WB)
R (TRITC: tetramethyl rhodamine iso-thiocyanate) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunohistochemistry (IHC)
TR (Texas Red®) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunohistochemistry (IHC)
PerCP (peridinin chlorophyll protein complex) and PerCP-Cy5.5 (peridinin chlorophyll protein complex with cyanin-5.5) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

mouse anti-rabbit IgG-CFL 790

sc-516253
200 µg/0.5 ml
$77.00

Would this AB work for immunofluorescence staining of cells?

Asked by: Patrycja
Thank you for your question. This secondary antibody is conjugated to CruzFluor™ 790 (CFL 790), which is a near infrared fluorescent dye. It could be used for immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
Answered by: Technical Support
Date published: 2019-05-25

做WESTERN BLOT,STAT3和EDG1都是兔来源一抗,扫膜用的是Odyssey的红外荧光成像,是否都可用sc-516253这款二抗?

Asked by: chenang
感谢您的提问。sc-516253 suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems,和一抗无关的。关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. sc-516253 suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-05-02
  • y_2025, m_12, d_8, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_516253, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 118ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great bandI received this antibody recently. I used it for western blot and I got a nice band.
Date published: 2017-08-31
  • y_2025, m_12, d_8, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_516253, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
mouse anti-rabbit IgG-CFL 790 is rated 5.0 out of 5 by 1.
  • y_2025, m_12, d_8, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_516253, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 131ms
  • REVIEWS, PRODUCT