Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MOF Double Nickase Plasmid (h): sc-401891-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MOF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MOF Double Nickase Plasmid (h) and MOF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KAT8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MOF Antibody (8C4C4): sc-81163
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOF Double Nickase Plasmid (h) | sc-401891-NIC | 20 µg | $410.00 | |||
MOF Double Nickase Plasmid (h2) | sc-401891-NIC-2 | 20 µg | $410.00 |
KAT8 encodes the histone acetyltransferase MOF (MYST1), a core writer of H4K16ac that modulates chromatin accessibility and transcriptional output. MOF functions within multiprotein complexes to coordinate gene expression programs linked to DNA replication, DNA damage signaling, and maintenance of genome stability, including crosstalk with ATM/DDR pathways. Through control of nucleosome architecture and acetylation-dependent recruitment of chromatin regulators, MOF influences cell-cycle progression, differentiation, and stress responses. Dysregulation of KAT8/MOF and altered H4K16 acetylation patterns have been associated with aberrant epigenetic states observed across multiple disease-relevant contexts, supporting its use as a mechanistic node in chromatin and genome integrity research.
MOF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KAT8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KAT8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KAT8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KAT8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.