MOF Antibody (T13H1B2): sc-81765

MOF Antibody (T13H1B2) is a mouse monoclonal IgG2b antibody that detects MOF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. MOF, also known as hMOF, MYST1, or MOZ, plays a crucial role in the regulation of gene expression by functioning as a histone acetyltransferase, which is essential for dosage compensation in organisms like Drosophila. MOF is primarily located in the nucleus, where MOF interacts with chromatin to modify histones, specifically acetylating histone H4 at lysine 16. This modification is vital as MOF facilitates the binding of the dosage compensation complex (DCC) to the X chromosome, thereby enhancing transcription levels in males who possess a single X chromosome. The unique structure of MOF, characterized by a C2HC-type zinc finger near its histone acetyltransferase domain, allows MOF to effectively engage with nucleosomes, ensuring proper gene regulation. Additionally, the carboxy terminal domain of MOF exhibits histone acetyltransferase activity towards histones H3 and H2A, further underscoring MOF′s importance in chromatin remodeling and gene expression regulation. With MOF′s ability to modulate histone acetylation, MOF is a key player in maintaining the balance of X-linked gene products between sexes, making MOF an important target for research in developmental biology and epigenetics.

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MOF Antibody (T13H1B2) References:

1. Modulations of hMOF autoacetylation by SIRT1 regulate hMOF recruitment and activities on the chromatin.  |  Lu, L., et al. 2011. Cell Res. 21: 1182-95. PMID: 21502975
2. Epigenetic change in kidney tumor: downregulation of histone acetyltransferase MYST1 in human renal cell carcinoma.  |  Wang, Y., et al. 2013. J Exp Clin Cancer Res. 32: 8. PMID: 23394073
3. The histone acetyltransferase hMOF suppresses hepatocellular carcinoma growth.  |  Zhang, J., et al. 2014. Biochem Biophys Res Commun. 452: 575-80. PMID: 25181338
4. Expression of hMOF, but not HDAC4, is responsible for the global histone H4K16 acetylation in gastric carcinoma.  |  Zhu, L., et al. 2015. Int J Oncol. 46: 2535-45. PMID: 25873202
5. Arsenic Trioxide Reduces Global Histone H4 Acetylation at Lysine 16 through Direct Binding to Histone Acetyltransferase hMOF in Human Cells.  |  Liu, D., et al. 2015. PLoS One. 10: e0141014. PMID: 26473953
6. Structural and Functional Role of Acetyltransferase hMOF K274 Autoacetylation.  |  McCullough, CE., et al. 2016. J Biol Chem. 291: 18190-8. PMID: 27382063
7. Glioma-induced SIRT1-dependent activation of hMOF histone H4 lysine 16 acetyltransferase in microglia promotes a tumor supporting phenotype.  |  Saidi, D., et al. 2018. Oncoimmunology. 7: e1382790. PMID: 29308302
8. The histone acetyltransferase hMOF promotes vascular invasion in hepatocellular carcinoma.  |  Poté, N., et al. 2020. Liver Int. 40: 956-967. PMID: 31943753
9. TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy.  |  Zehender, A., et al. 2021. Nat Commun. 12: 4404. PMID: 34285225
10. hMOF induces cisplatin resistance of ovarian cancer by regulating the stability and expression of MDM2.  |  Cai, M., et al. 2023. Cell Death Discov. 9: 179. PMID: 37291112

The preferred MOF antibody is MOF Antibody (8C4C4): sc-81163.