MOB1B CRISPR Activation Plasmid (h): sc-416280-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MOB1B CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- MOB1B CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by MOB1B CRISPR Activation Plasmid (h) and MOB1B CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the MOB1B transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOB1B CRISPR Activation Plasmid (h) | sc-416280-ACT | 20 µg | $397.00 | |||
MOB1B CRISPR Activation Plasmid (h2) | sc-416280-ACT-2 | 20 µg | $397.00 |
MOB1B (MOB kinase activator 1B) encodes a conserved co-activator of LATS1/2 kinases within the Hippo signaling pathway, supporting phosphorylation cascades that regulate YAP/TAZ activity, transcriptional programs, and contact-dependent growth control. Through Hippo pathway modulation, MOB1B contributes to cell cycle progression, apoptosis, and maintenance of tissue homeostasis. Perturbation of Hippo components, including MOB family regulators, is linked to altered proliferation and migration phenotypes that are frequently studied in the context of oncogenic signaling and epithelial integrity. MOB1B is therefore relevant for investigating pathway cross-talk with MAPK, PI3K-AKT, and cytoskeletal remodeling networks that influence cell fate decisions.
MOB1B CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous MOB1B expression without altering the underlying DNA sequence.
MOB1B CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the MOB1B locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the MOB1B transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous MOB1B expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native MOB1B locus and enabling the study of MOB1B-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of MOB1B pathway restoration in tumor cells with silenced or reduced MOB1B expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.