Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mnk2 Double Nickase Plasmid (h): sc-401879-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mnk2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mnk2 Double Nickase Plasmid (h) and Mnk2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MKNK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mnk2 Antibody (B-6): sc-271559
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mnk2 Double Nickase Plasmid (h) | sc-401879-NIC | 20 µg | $410.00 | |||
Mnk2 Double Nickase Plasmid (h2) | sc-401879-NIC-2 | 20 µg | $410.00 |
MKNK2 encodes MAP kinase–interacting serine/threonine-protein kinase 2 (Mnk2), a downstream effector of ERK and p38 MAPK signaling that couples mitogen and stress inputs to control of mRNA translation. Mnk2 phosphorylates eIF4E at Ser209, influencing cap-dependent translation initiation and selective translation of transcripts involved in growth, inflammation, and stress responses. Through integration with the MAPK cascade and translational control machinery, Mnk2 contributes to regulation of proliferation, survival, and cytokine-driven signaling programs. Dysregulated MNK–eIF4E signaling has been associated with altered oncogenic and inflammatory gene expression patterns, making MKNK2 a relevant node for mechanistic studies of disease-linked translation control.
Mnk2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MKNK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MKNK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MKNK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MKNK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.