MMP2 Double Nickase Plasmid (m): sc-421674-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMP2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MMP2 Double Nickase Plasmid (m) and MMP2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mmp2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MMP2 Antibody (2C1): sc-13594
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMP2 Double Nickase Plasmid (m) | sc-421674-NIC | 20 µg | $410.00 | |||
MMP2 Double Nickase Plasmid (m2) | sc-421674-NIC-2 | 20 µg | $410.00 |
Mouse Mmp2 encodes matrix metalloproteinase-2 (MMP2), a secreted zinc-dependent endopeptidase that degrades basement membrane and interstitial extracellular matrix components such as type IV collagen and gelatin. MMP2 activity is regulated through secretion as a zymogen and activation at the cell surface in coordination with MT1-MMP (MMP14) and TIMP2, linking it to pericellular proteolysis, cell migration, and tissue remodeling. Through extracellular matrix turnover, MMP2 intersects with integrin signaling, epithelial–mesenchymal transition programs, and inflammatory remodeling pathways. Dysregulated MMP2 has been associated with fibrotic remodeling, vascular and cardiac matrix alterations, and invasive phenotypes in oncology models, making it a common target for mechanistic studies in mouse systems.
MMP2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mmp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mmp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mmp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mmp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.