MMP2 Double Nickase Plasmid (h): sc-400126-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MMP2 Double Nickase Plasmid (h) and MMP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MMP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MMP2 Antibody (2C1): sc-13594
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMP2 Double Nickase Plasmid (h) | sc-400126-NIC | 20 µg | $410.00 | |||
MMP2 Double Nickase Plasmid (h2) | sc-400126-NIC-2 | 20 µg | $410.00 |
Human MMP2 encodes matrix metalloproteinase-2 (gelatinase A), a zinc-dependent endopeptidase that degrades type IV collagen and other extracellular matrix substrates, thereby regulating basement membrane remodeling and cell–matrix interactions. MMP2 activity is controlled by secretion as a zymogen, activation at the cell surface, and inhibition by TIMPs, linking it to pericellular proteolysis and dynamic changes in tissue architecture. Through ECM turnover, MMP2 influences angiogenesis, wound repair, inflammation, and cell migration pathways that depend on integrin signaling and protease cascades. Dysregulated MMP2 expression or activity is associated with pathological matrix remodeling observed in cancer invasion and metastasis, cardiovascular remodeling, and fibrotic and inflammatory conditions, making it a key target for mechanistic studies of tissue microenvironment regulation.
MMP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MMP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MMP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MMP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MMP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.