MM-142 + PMA nuclear extract: sc-2140

MM-142 + PMA nuclear extract is a highly specialized reagent used in molecular biology research to study signal transduction and gene expression regulation within cellular nuclei. This extract combines MM-142, a nuclear extract rich in transcription factors and nuclear proteins, with Phorbol 12-myristate 13-acetate (PMA), a diester of phorbol known to activate protein kinase C (PKC). The addition of PMA to MM-142 enhances the analysis of PKC-mediated pathways and their impact on nuclear function, particularly in the context of transcriptional regulation and chromatin remodeling. Researchers utilize this composite nuclear extract to investigate the dynamics of PKC activation in nuclear signaling, exploring how PKC influences transcription factors, modifies chromatin structure, and affects gene expression profiles. The dual components of MM-142 + PMA allow for a more robust examination of cellular responses to PKC activators in a controlled in vitro environment, providing insights into the complex interactions within the nucleus that govern cell behavior. Verification of the cell line origin used for extracting MM-142 ensures the reproducibility and relevance of research findings, emphasizing the applications of this biochemical tool.