MIXL1 Antibody (E-10): sc-518255

MIXL1 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects an epitope mapping between amino acids 100-121 of MIXL1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MIXL1 antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIXL1, also known as Mix1 homeobox-like 1, is a 232 amino acid transcription factor that plays a crucial role in axial mesendoderm morphogenesis and endoderm formation, which are essential processes during embryonic development. Located primarily in the nucleus, MIXL1 contains a highly conserved homeobox DNA-binding domain that enables regulation of gene expression through its helix-turn-helix structure. This structural feature allows MIXL1 to bind to specific DNA sequences and modulate the transcription of target genes, influencing cellular differentiation, particularly in blood development and the maturation of heart and gut tissues. MIXL1′s involvement in the negative regulation of brachyury expression highlights its significance in developmental biology. Notably, overexpression of MIXL1 has been linked to non-Hodgkin and Hodgkin lymphomas, indicating potential involvement in carcinogenesis and making MIXL1 a valuable target for research in cancer biology. These attributes establish MIXL1 (E-10) monoclonal antibody as an essential tool for investigating developmental processes and cancer biology implications.

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MIXL1 Antibody (E-10) References:

1. A human Mix-like homeobox gene MIXL shows functional similarity to Xenopus Mix.1.  |  Guo, W., et al. 2002. Blood. 100: 89-95. PMID: 12070013
2. Structure, upstream promoter region, and functional domains of a mouse and human Mix paired-like homeobox gene.  |  Sahr, K., et al. 2002. Gene. 291: 135-47. PMID: 12095687
3. Mixl1 is required for axial mesendoderm morphogenesis and patterning in the murine embryo.  |  Hart, AH., et al. 2002. Development. 129: 3597-608. PMID: 12117810
4. Transcriptional regulation of the homeobox gene Mixl1 by TGF-beta and FoxH1.  |  Hart, AH., et al. 2005. Biochem Biophys Res Commun. 333: 1361-9. PMID: 15982639
5. Differential expression of the human MIXL1 gene product in non-Hodgkin and Hodgkin lymphomas.  |  Drakos, E., et al. 2007. Hum Pathol. 38: 500-7. PMID: 17303500
6. Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors.  |  Davis, RP., et al. 2008. Blood. 111: 1876-84. PMID: 18032708
7. The preleukemic state of mice reconstituted with Mixl1-transduced marrow cells.  |  Metcalf, D., et al. 2007. Proc Natl Acad Sci U S A. 104: 20013-8. PMID: 18056627
8. Enforced expression of Mixl1 during mouse ES cell differentiation suppresses hematopoietic mesoderm and promotes endoderm formation.  |  Lim, SM., et al. 2009. Stem Cells. 27: 363-74. PMID: 19038793
9. Pdgfrα and Flk1 are direct target genes of Mixl1 in differentiating embryonic stem cells.  |  Pereira, LA., et al. 2012. Stem Cell Res. 8: 165-79. PMID: 22265737
10. A conditional mutant allele for analysis of Mixl1 function in the mouse.  |  Pulina, MV., et al. 2014. Genesis. 52: 417-23. PMID: 24596343
11. Mixl1 localizes to putative axial stem cell reservoirs and their posterior descendants in the mouse embryo.  |  Wolfe, AD. and Downs, KM. 2014. Gene Expr Patterns. 15: 8-20. PMID: 24632399

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MIXL1 Antibody (E-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MIXL1 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MIXL1 Antibody (E-10) Specifications

Alternate Product Name: MIXL1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 100-121

Epitope Target: MIXL1

Epitope Species: human

Product Reactivity/Detection: MIXL1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MIXL1 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin