Mito Red CAS: 1021902-10-2
MF: C38H37N2O5•Cl
MW: 637.16
A cell membrane permeable rhodamine based dye.

Mito Red (CAS 1021902-10-2)

Mito Red | CAS 1021902-10-2 is rated 5.0 out of 5 by 1.
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Alternate Names: 3,6-Bis(diethylamino)-9-[2-(4-methylcoumarin-7-yloxycarbonyl)phenyl]xanthylium chloride
Application: Mito Red is a cell membrane permeable rhodamine based dye
CAS Number: 1021902-10-2
Purity: ≥70%
Molecular Weight: 637.16
Molecular Formula: C38H37N2O5•Cl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Mito Red is a cell membrane permeable rhodamine based dye. It localizes mitochondria, and emits red fluorescence. The interaction of Mito Red with mitochondria depends on the membrane potential of the mitochondria. Mitochondria can be stained with 20-200 nM of Mito Red.

Usage :
Staining Mitochondria


• Prepare 1mM Mito Red DMSO solution and incubate at 37°C prior to use.

• Prepare cells on glass slide, 5x104 to 5x105 cells.

•Dilute 1mM Mito Red DMSO solution in culture media to 20-200nM.

•Add diluted solution to slide and incubate at 37°C for 30-60 min.

•Remove solution and wash cells with culture medium.

•OPTIONAL: to fix cells add 10% formarin buffer and incubate for 15-20 min followed with washing with PBS.

•Observe slide under fluorescence microscope with rhodamine filter.




Physical State :
Solid
Solubility :
Soluble in DMSO (1 mM) = 50 µg (1tube) in 78 µL of provided DMSO
Storage :
Store at 4° C
Fluoresence :
λex 569 nm, λem 594 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
24963097
MDL Number :
MFCD05664720
SMILES :
CCN(CC)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](CC)CC)C=C3O2)C4=CC=CC=C4C(=O)OC5=CC6=C(C=C5)C(=CC(=O)O6)C.[Cl-]

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Certificate of Analysis

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Mito Red (CAS 1021902-10-2) Product Citations

See how others have used Mito Red (CAS 1021902-10-2). Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 28752496  Ding, M. et al. 2017. Cytotechnology.

PMID: # 27000946  Pandurangan, M. et al. 2016. J. Mol. Recognit. 29: 426-35.

PMID: # 26811107  Pandurangan, M. et al. 2016. Biol Trace Elem Res. 173: 62-70.

PMID: # 27424190  Lee, YH. et al. 2016. J. Cancer Res. Clin. Oncol. 142: 1967-77.

Citations 1 to 4 of 4 total

Could you please provide me a sample pack of Mito-Red (Cat No. sc-301164)? So that we can purchase it in near future.

Asked by: ARINDAM BISWAS
Thank you for your question. We do not provide sample sizes for our research chemicals. The smallest available size is advertised on our website.
Answered by: Technical Support
Date published: 2017-07-28
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Rated 5 out of 5 by from Mito red is a rhodamine Mito red is a rhodamine-based, fluorescent red dye that localizes mitochondria and its use has been cited in various publications. -SCBT Publication Review
Date published: 2015-03-24
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