Mito Red is a cell membrane permeable rhodamine based dye. It localizes mitochondria, and emits red fluorescence. The interaction of Mito Red with mitochondria depends on the membrane potential of the mitochondria. Mitochondria can be stained with 20-200 nM of Mito Red.
• Prepare 1mM Mito Red DMSO solution and incubate at 37°C prior to use.
• Prepare cells on glass slide, 5x104 to 5x105 cells.
•Dilute 1mM Mito Red DMSO solution in culture media to 20-200nM.
•Add diluted solution to slide and incubate at 37°C for 30-60 min.
•Remove solution and wash cells with culture medium.
•OPTIONAL: to fix cells add 10% formarin buffer and incubate for 15-20 min followed with washing with PBS.
•Observe slide under fluorescence microscope with rhodamine filter.
Soluble in DMSO (1 mM) = 50 µg (1tube) in 78 µL of provided DMSO
Store at 4° C
λex 569 nm, λem 594 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Rated 5 out of
Mito red is a rhodamineMito red is a rhodamine-based, fluorescent red dye that localizes mitochondria and its use has been cited in various publications. -SCBT Publication Review
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