Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mimitin Double Nickase Plasmid (h): sc-410509-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mimitin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mimitin Double Nickase Plasmid (h) and Mimitin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NDUFAF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mimitin Antibody (H-11): sc-365592
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mimitin Double Nickase Plasmid (h) | sc-410509-NIC | 20 µg | $410.00 | |||
Mimitin Double Nickase Plasmid (h2) | sc-410509-NIC-2 | 20 µg | $410.00 |
NDUFAF2 (Mimitin) is a mitochondrial matrix assembly factor required for proper biogenesis of respiratory chain complex I (NADH:ubiquinone oxidoreductase). By supporting complex I maturation and stability, Mimitin contributes to oxidative phosphorylation, maintenance of mitochondrial membrane potential, and control of cellular redox balance that influences metabolic and stress-response pathways. Perturbation of complex I assembly can elevate reactive oxygen species and compromise ATP production, linking NDUFAF2 dysfunction to mitochondrial pathophysiology and energy-demanding tissues. As a nuclear-encoded regulator of mitochondrial function, NDUFAF2 is frequently studied in contexts of metabolic remodeling, mitochondrial quality control, and mechanisms underlying complex I–associated disorders.
Mimitin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NDUFAF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NDUFAF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NDUFAF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NDUFAF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.