MIC2 Antibody (12E7): sc-53148

MIC2 Antibody (12E7) is a mouse monoclonal IgG1 kappa light chain antibody that detects the MIC2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MIC2 antibody (12E7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIC2 protein, also known as CD99, is a critical T cell surface protein that plays a significant role in lymphocyte aggregation and adhesion. CD99 exists in two forms produced by alternative splicing: the major form promotes cellular adhesion, while the truncated form inhibits this process. This regulation of adhesion is vital, as CD99 modulates the interaction between lymphocytes and the intercellular adhesion molecule-1 (ICAM-1) through lymphocyte function-associated antigen-1 (LFA-1). Notably, overexpression of the truncated form of CD99 leads to a downregulation of LFA-1, which is associated with the Hodgkin′s and Reed-Sternberg (H-RS) phenotype in certain cells, underscoring the importance of CD99 in maintaining proper cell function and morphology. MIC2 (12E7) antibody effectively detects and characterizes this protein, making MIC2 (12E7) antibody an invaluable tool for researchers studying immune responses and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MIC2 Antibody (12E7) References:

1. A role for CD99 in T cell activation.  |  Wingett, D., et al. 1999. Cell Immunol. 193: 17-23. PMID: 10202109
2. MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Evidence for a common histogenesis of Ewing's sarcoma and peripheral primitive neuroectodermal tumors from MIC2 expression and specific chromosome aberration.  |  Ambros, IM., et al. 1991. Cancer. 67: 1886-93. PMID: 1848471
3. [Co-expression of CD99/MIC2 and ALK in anaplastic large-cell lymphoma tissues and its significance].  |  Zhong, L., et al. 2012. Zhonghua Xue Ye Xue Za Zhi. 33: 173-6. PMID: 22781601
4. The MIC2 gene product: epitope mapping and structural prediction analysis define an integral membrane protein.  |  Banting, GS., et al. 1989. Mol Immunol. 26: 181-8. PMID: 2465491
5. The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product.  |  Gelin, C., et al. 1989. EMBO J. 8: 3253-9. PMID: 2479542
6. A novel method for producing anti-peptide antibodies. Production of site-specific antibodies to the T cell antigen receptor beta-chain.  |  Posnett, DN., et al. 1988. J Biol Chem. 263: 1719-25. PMID: 3276675
7. Immunohistochemical profile of monoclonal antibody O13: antibody that recognizes glycoprotein p30/32MIC2 and is useful in diagnosing Ewing's sarcoma and peripheral neuroepithelioma.  |  Weidner, N. and Tjoe, J. 1994. Am J Surg Pathol. 18: 486-94. PMID: 7513503
8. MIC2 analysis of small cell carcinoma.  |  Lumadue, JA., et al. 1994. Am J Clin Pathol. 102: 692-4. PMID: 7524308
9. Microwave oven antigen retrieval applied to the immunostaining of cytopathology specimens.  |  Reynolds, GM., et al. 1994. Cytopathology. 5: 345-58. PMID: 7533548
10. CD99 (MIC2) regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes, and its gene encodes both positive and negative regulators of cellular adhesion.  |  Hahn, JH., et al. 1997. J Immunol. 159: 2250-8. PMID: 9278313
11. Engagement of CD99 induces up-regulation of TCR and MHC class I and II molecules on the surface of human thymocytes.  |  Choi, EY., et al. 1998. J Immunol. 161: 749-54. PMID: 9670951
12. Generation of cells with Hodgkin's and Reed-Sternberg phenotype through downregulation of CD99 (Mic2).  |  Kim, SH., et al. 1998. Blood. 92: 4287-95. PMID: 9834235

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MIC2 Antibody (12E7) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-MIC2 Antibody (12E7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MIC2 Antibody (12E7) Specifications

Alternate Product Name: MIC2

Product Clone Name: 12E7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MIC2

Epitope Species: human

Product Reactivity/Detection: MIC2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for MIC2 Antibody (12E7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin