Mi2 Antibody (B-4): sc-55606

Mi2 Antibody (B-4) is a mouse monoclonal IgA kappa light chain antibody that detects Mi2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Mi2 antibody (B-4) is available as a non-conjugated form, providing versatility for various experimental needs. Mi2 protein plays a crucial role in chromatin remodeling, essential for gene expression regulation. Mi2 (B-4) monoclonal antibody is part of the mSin3 corepressor complex, involved in histone deacetylation, leading to transcriptional repression. This complex includes several key components such as mSin3, histone deacetylases HDAC1 and HDAC2, and autoantigens Mi2-α and Mi2-β. Mi2 protein′s interaction with these components highlights its importance in maintaining chromatin structure and function, as alterations in chromatin can significantly impact cellular processes, including differentiation and response to environmental signals. Mi2 (B-4) antibody enables researchers to gain insights into transcriptional regulation mechanisms and chromatin dynamics in various biological contexts.

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Mi2 Antibody (B-4) References:

1. The TWIST/Mi2/NuRD protein complex and its essential role in cancer metastasis.  |  Fu, J., et al. 2011. Cell Res. 21: 275-89. PMID: 20714342
2. More prominent muscle involvement in patients with dermatomyositis with anti-Mi2 autoantibodies.  |  Pinal-Fernandez, I., et al. 2019. Neurology. 93: e1768-e1777. PMID: 31594859
3. Severe muscle damage with myofiber necrosis and macrophage infiltrates characterize anti-Mi2 positive dermatomyositis.  |  Fornaro, M., et al. 2021. Rheumatology (Oxford). 60: 2916-2926. PMID: 33249503
4. Transcriptional derepression of CHD4/NuRD-regulated genes in the muscle of patients with dermatomyositis and anti-Mi2 autoantibodies.  |  Pinal-Fernandez, I., et al. 2023. Ann Rheum Dis. 82: 1091-1097. PMID: 37130727
5. The major dermatomyositis-specific Mi-2 autoantigen is a presumed helicase involved in transcriptional activation.  |  Seelig, HP., et al. 1995. Arthritis Rheum. 38: 1389-99. PMID: 7575689
6. Nucleosome structural changes due to acetylation.  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
7. A positive role for histone acetylation in transcription factor access to nucleosomal DNA.  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
8. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
9. Repression and activation by multiprotein complexes that alter chromatin structure.  |  Kingston, RE., et al. 1996. Genes Dev. 10: 905-20. PMID: 8608939
10. Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex.  |  Zhang, Y., et al. 1997. Cell. 89: 357-64. PMID: 9150135
11. SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex.  |  Zhang, Y., et al. 1998. Mol Cell. 1: 1021-31. PMID: 9651585
12. The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities.  |  Zhang, Y., et al. 1998. Cell. 95: 279-89. PMID: 9790534

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mi2 Antibody (B-4) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Mi2 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mi2 Antibody (B-4) Specifications

Alternate Product Name: Mi2

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgA kappa light chain

Amino Acids: 1671-1912

Epitope Target: Mi2

Epitope Species: human

Product Reactivity/Detection: Mi2-α and Mi2-β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgA kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Mi2 Antibody (B-4): N/A