MGC13057 Double Nickase Plasmid (h): sc-413610-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MGC13057 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MGC13057 Double Nickase Plasmid (h) and MGC13057 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C2orf88. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGC13057 Double Nickase Plasmid (h) | sc-413610-NIC | 20 µg | $410.00 | |||
MGC13057 Double Nickase Plasmid (h2) | sc-413610-NIC-2 | 20 µg | $410.00 |
C2orf88 encodes the poorly characterized protein MGC13057, which has been reported as a context-dependent regulator of epithelial cell state and differentiation-associated transcriptional programs. Expression patterns link C2orf88 to pathways governing cell adhesion, cytoskeletal organization, and microenvironmental signaling, suggesting roles in maintaining tissue architecture and modulating stress-responsive gene expression. Altered C2orf88 levels have been observed in multiple tumor datasets and inflammatory contexts, supporting its use as a biomarker-like gene for studying mechanisms that connect epithelial plasticity with disease-relevant phenotypes. Functional perturbation of MGC13057 can help define upstream regulators and downstream effectors within these remodeling and differentiation networks.
MGC13057 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C2orf88 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C2orf88. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C2orf88 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C2orf88-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.