METTL11B Double Nickase Plasmid (h): sc-414653-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- METTL11B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- METTL11B Double Nickase Plasmid (h) and METTL11B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting METTL11B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
METTL11B Double Nickase Plasmid (h) | sc-414653-NIC | 20 µg | $410.00 | |||
METTL11B Double Nickase Plasmid (h2) | sc-414653-NIC-2 | 20 µg | $410.00 |
METTL11B encodes a putative S-adenosylmethionine–dependent methyltransferase implicated in protein methylation and broader epigenetic-like regulation of proteome function. As a member of methyltransferase-like enzymes, METTL11B is studied for its potential roles in modulating protein stability, subcellular localization, and interaction networks that influence cell growth and stress responses. Dysregulated methylation-associated pathways are frequently linked to altered transcriptional programs and aberrant signaling in cancer and neurodevelopmental contexts, making METTL11B a gene of interest for mechanistic studies. Ongoing functional annotation efforts aim to clarify its substrates and contribution to conserved methylation-dependent cellular processes in human cells.
METTL11B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the METTL11B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within METTL11B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt METTL11B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of METTL11B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.