Metallothionein 1F Double Nickase Plasmid (h): sc-417937-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Metallothionein 1F Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Metallothionein 1F Double Nickase Plasmid (h) and Metallothionein 1F Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MT1F. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Metallothionein 1F Double Nickase Plasmid (h) | sc-417937-NIC | 20 µg | $410.00 | |||
Metallothionein 1F Double Nickase Plasmid (h2) | sc-417937-NIC-2 | 20 µg | $410.00 |
MT1F encodes metallothionein 1F, a low–molecular weight, cysteine-rich metal-binding protein that contributes to intracellular homeostasis of essential trace metals such as zinc and copper and sequestration of toxic metals including cadmium and mercury. By buffering labile zinc pools, MT1F can influence redox balance, metal-dependent enzyme activity, and transcriptional programs responsive to oxidative and electrophilic stress. Metallothionein family members are commonly linked to cellular stress adaptation pathways and can modulate sensitivity to metal exposure and reactive oxygen species through thiol-based redox chemistry. Altered expression of MT1F has been reported across multiple disease-relevant contexts, including cancer-associated changes in metal metabolism and oxidative stress signaling, making it a useful target for mechanistic studies of stress responses and detoxification biology.
Metallothionein 1F Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MT1F locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MT1F. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MT1F function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MT1F-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.