Metallothionein 1A CRISPR Activation Plasmid (h): sc-410887-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Metallothionein 1A CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Metallothionein 1A CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Metallothionein 1A CRISPR Activation Plasmid (h) and Metallothionein 1A CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the MT1A transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Metallothionein 1A CRISPR Activation Plasmid (h) | sc-410887-ACT | 20 µg | $397.00 | |||
Metallothionein 1A CRISPR Activation Plasmid (h2) | sc-410887-ACT-2 | 20 µg | $397.00 |
MT1A encodes metallothionein 1A, a cysteine-rich metal-binding protein that buffers intracellular zinc and copper and sequesters toxic heavy metals such as cadmium and mercury. By regulating metal homeostasis and cellular redox balance, MT1A influences oxidative stress responses, detoxification programs, and signaling pathways that depend on zinc availability, including transcription factor activity and stress-inducible gene networks. MT1A expression is responsive to metal exposure, inflammatory cues, and cellular stress, and altered metallothionein regulation has been associated with susceptibility to metal toxicity, tissue injury, and broader pathophysiology linked to oxidative damage. These properties make MT1A a useful node for studying metal-dependent proteostasis, stress adaptation, and interactions between detoxification and inflammatory signaling.
Metallothionein 1A CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous MT1A expression without altering the underlying DNA sequence.
Metallothionein 1A CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the MT1A locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the MT1A transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Metallothionein 1A expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native MT1A locus and enabling the study of Metallothionein 1A-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Metallothionein 1A pathway restoration in tumor cells with silenced or reduced MT1A expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.