MESDC2 Double Nickase Plasmid (h): sc-406189-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MESDC2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MESDC2 Double Nickase Plasmid (h) and MESDC2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MESDC2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MESDC2 Double Nickase Plasmid (h) | sc-406189-NIC | 20 µg | $410.00 | |||
MESDC2 Double Nickase Plasmid (h2) | sc-406189-NIC-2 | 20 µg | $410.00 |
MESDC2 (mesoderm development candidate 2) encodes an endoplasmic reticulum–resident chaperone that partners with LRPAP1 to support folding, maturation, and ER export of LDL receptor–related proteins, particularly LRP5 and LRP6. By enabling proper assembly and trafficking of these Wnt co-receptors, MESDC2 influences canonical Wnt/β-catenin signaling and downstream programs governing cell fate decisions, development, and tissue homeostasis. Perturbation of MESDC2 function is therefore relevant to research on Wnt pathway dysregulation, including contexts such as developmental anomalies and cancer-associated signaling rewiring. Its role in quality control of secretory pathway receptors also makes MESDC2 a useful node for studying ER proteostasis and receptor biogenesis.
MESDC2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MESDC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MESDC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MESDC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MESDC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.