MES monohydrate (CAS 145224-94-8)

MES monohydrate, a zwitterionic N-substituted aminosulfonic acid Good′s buffer, holds significant importance as an organic acid and a widely utilized buffer in biochemical and physiological research. This versatile compound plays various roles in laboratory experiments and biological systems. In scientific research, MES monohydrate finds extensive application, including protein purification, enzyme assays, and cell culture media. It is a preferred choice for protein purification due to its low ionic strength and its ability to adjust the pH of the solution effectively. Enzyme assays benefit from MES monohydrate as it helps maintain a stable pH in the reaction mixture. Moreover, in cell culture media, MES monohydrate serves the purpose of pH maintenance and provides a buffering effect for the cultured cells. MES monohydrate′s zwitterionic nature, combining positively charged morpholino groups with negatively charged ethanesulfonate groups, enables it to act as a reliable buffer in biological systems. Its unique charge distribution allows it to bind to positively and negatively charged molecules, ensuring a constant pH even in the presence of acid or base molecules. Furthermore, MES monohydrate has specific applications in protein crystallography, where it acts as a buffering agent. It is also a suitable running buffer for resolving small proteins, such as 13 kDa, on bis-tris gels. Additionally, it serves as a biological buffer in plant cell cultures, facilitating optimal growth conditions.