MES is a zwitterionic N-substituted aminosulfonic acid known as a Good′s buffer, active in the pH range 6-8 with a pKa of 6.10 at 25°C. With a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. MES is used as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide.
Also available: MES, Sodium Salt (sc-216095); MES hemisodium salt (sc-216093); MES solution (sc-300959); MES monohydrate (sc-255262)
1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950 2. Graf, H., et al. 1994. Bioseparation. 4: 7-20. PMID: 7764588 3. Anfossi, L., et al. 2003. Ann Chim. 93: 499-512. PMID: 12911143 4. Mönch, S., et al. 2010. Anal. Biochem. 398: 150-160. PMID: 19903444 5. Teste, B., et al. 2010. Talanta. 81: 1703-1710. PMID: 20441961
Filter solution through a 0.22 μm filter into a sterile flask. Solutions are stable when stored at 2-8°C for several months. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving.
Soluble in water (~1.3 M).
useful buffering range: pH 5.5 - 6.7
Store at room temperature
1.4 g/cm3 (Predicted)
n20D 1.52 (Predicted)
pKa: 6.1 at 25°C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Various publications cite the use of MESVarious publications cite the use of MES, a zwitterionic buffer, as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide. -SCBT Publication Review
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