MEF-2D Double Nickase Plasmid (h): sc-403475-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MEF-2D Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MEF-2D Double Nickase Plasmid (h) and MEF-2D Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MEF2D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MEF-2D Antibody (H-11): sc-271153
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MEF-2D Double Nickase Plasmid (h) | sc-403475-NIC | 20 µg | $410.00 | |||
MEF-2D Double Nickase Plasmid (h2) | sc-403475-NIC-2 | 20 µg | $410.00 |
MEF2D encodes the human transcription factor MEF-2D, a MADS-box/MEF2 family DNA-binding protein that integrates calcium-dependent signaling with context-specific gene expression programs. MEF-2D regulates differentiation and adaptive transcription in multiple lineages, including myogenic and neuronal states, by cooperating with cofactors such as class IIa HDACs and responding to MAPK and calcineurin pathways. Through these signaling-responsive interactions, MEF-2D helps coordinate chromatin state and transcriptional outputs that shape cell-cycle control, survival, and lineage identity. Dysregulated MEF2D activity and altered MEF2D-driven transcriptional networks have been implicated in cancer-associated transcriptional reprogramming and hematologic malignancy genetics, supporting its use as a functional node in pathway and disease-mechanism studies.
MEF-2D Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MEF2D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MEF2D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MEF2D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MEF2D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.