MEF-2C Double Nickase Plasmid (m): sc-421620-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MEF-2C Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MEF-2C Double Nickase Plasmid (m) and MEF-2C Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mef2c. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MEF-2C Antibody (G-5): sc-518152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MEF-2C Double Nickase Plasmid (m) | sc-421620-NIC | 20 µg | $410.00 | |||
MEF-2C Double Nickase Plasmid (m2) | sc-421620-NIC-2 | 20 µg | $410.00 |
Mef2c encodes the transcription factor MEF-2C, a MADS-box/MEF2 family regulator that integrates Ca2+-dependent signaling to control lineage-specific gene programs. MEF-2C coordinates chromatin and transcriptional networks involved in myogenesis, neuronal differentiation and synaptic remodeling, immune cell development, and cardiogenesis through interactions with cofactors such as class IIa HDACs and MAPK/CaMK-responsive pathways. In mouse models, altered Mef2c dosage or activity perturbs developmental patterning and activity-dependent transcription, making it relevant to studies of neurodevelopmental phenotypes, cardiac conduction and remodeling, and hematopoietic lineage specification. Its broad regulatory scope also links MEF-2C to stress-response and differentiation programs that can be interrogated across primary cells and stem cell-derived systems.
MEF-2C Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mef2c locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mef2c. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mef2c function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mef2c-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.