Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ME1 Double Nickase Plasmid (h): sc-401587-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ME1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ME1 Double Nickase Plasmid (h) and ME1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ME1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ME1 Antibody (C-6): sc-365891
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ME1 Double Nickase Plasmid (h) | sc-401587-NIC | 20 µg | $410.00 | |||
ME1 Double Nickase Plasmid (h2) | sc-401587-NIC-2 | 20 µg | $410.00 |
Human ME1 encodes cytosolic NADP-dependent malic enzyme 1, which catalyzes the oxidative decarboxylation of malate to pyruvate while generating NADPH. This activity supports reductive biosynthesis and redox homeostasis by supplying NADPH for fatty acid and cholesterol synthesis and for antioxidant systems such as glutathione and thioredoxin. ME1 function integrates with central carbon metabolism, linking glycolysis, the tricarboxylic acid cycle, and anaplerotic fluxes that influence cellular energy balance. Dysregulated ME1 expression or activity has been associated with metabolic reprogramming in proliferative states and with altered lipid metabolism and oxidative stress phenotypes relevant to cancer and metabolic disease research.
ME1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ME1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ME1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ME1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ME1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.