Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MCT4 Double Nickase Plasmid (h): sc-418517-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCT4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MCT4 Double Nickase Plasmid (h) and MCT4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC16A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MCT4 Antibody (D-1): sc-376140
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCT4 Double Nickase Plasmid (h) | sc-418517-NIC | 20 µg | $410.00 | |||
MCT4 Double Nickase Plasmid (h2) | sc-418517-NIC-2 | 20 µg | $410.00 |
SLC16A3 encodes monocarboxylate transporter 4 (MCT4), a plasma membrane H+-coupled symporter that exports lactate and other monocarboxylates to regulate intracellular pH and sustain glycolytic metabolism. MCT4 functions in metabolic reprogramming by supporting rapid NAD+ regeneration and limiting acid stress, integrating with hypoxia and HIF-1–associated transcriptional programs. Its activity shapes the extracellular microenvironment through lactate efflux, influencing redox balance, nutrient partitioning, and cell–cell metabolic coupling. Dysregulated SLC16A3/MCT4 expression is associated with high-glycolysis states and has been implicated in cancer metabolism and other disorders linked to altered lactate handling and pH homeostasis.
MCT4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC16A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC16A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC16A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC16A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.