MC5-R Double Nickase Plasmid (h): sc-403844-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MC5-R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MC5-R Double Nickase Plasmid (h) and MC5-R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MC5R. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MC5-R Double Nickase Plasmid (h) | sc-403844-NIC | 20 µg | $410.00 | |||
MC5-R Double Nickase Plasmid (h2) | sc-403844-NIC-2 | 20 µg | $410.00 |
Human MC5R encodes melanocortin receptor 5 (MC5-R), a G protein–coupled receptor activated by melanocortin peptides such as α-MSH and ACTH. MC5-R primarily signals through Gs to elevate intracellular cAMP and engage PKA/CREB-dependent transcriptional programs, with downstream effects on cellular metabolism, secretory activity, and immune modulation in a tissue-dependent manner. MC5R activity has been linked to regulation of exocrine gland function, lipid handling, and inflammatory signaling, and altered melanocortin pathway tone is studied in the context of metabolic and immune-associated disorders. Because melanocortin receptors integrate neuroendocrine cues with peripheral responses, MC5R serves as a useful node for dissecting GPCR signaling dynamics and cAMP-controlled gene networks.
MC5-R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MC5R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MC5R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MC5R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MC5R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.