MBD2 Antibody (C-11): sc-514062

MBD2 Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects MBD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MBD2 (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MBD2 plays a crucial role in gene expression regulation through DNA methylation, a key epigenetic modification that influences transcriptional activity. MBD2 binds to methylated DNA, particularly at CpG sites, and recruits histone deacetylases, which contribute to transcription repression. This function maintains cellular identity and regulates developmental processes, as DNA methylation pattern abnormalities can lead to various diseases, including cancer. MBD2 belongs to a larger family of methyl-CpG binding proteins, including MBD1, MBD3, MBD4, and MeCP2, which interact with methylated DNA and contribute to gene silencing. MBD proteins show notable expression in somatic tissues, highlighting their significance in tissue-specific gene regulation.

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MBD2 Antibody (C-11) References:

1. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes.  |  Hendrich, B., et al. 1999. Mamm Genome. 10: 906-12. PMID: 10441743
2. Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.  |  Fujita, N., et al. 1999. Mol Cell Biol. 19: 6415-26. PMID: 10454587
3. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex.  |  Ng, HH., et al. 1999. Nat Genet. 23: 58-61. PMID: 10471499
4. The thymine glycosylase MBD4 can bind to the product of deamination at methylated CpG sites.  |  Hendrich, B., et al. 1999. Nature. 401: 301-4. PMID: 10499592
5. Solution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1.  |  Ohki, I., et al. 1999. EMBO J. 18: 6653-61. PMID: 10581239
6. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein.  |  Boyes, J. and Bird, A. 1991. Cell. 64: 1123-34. PMID: 2004419
7. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex.  |  Nan, X., et al. 1998. Nature. 393: 386-9. PMID: 9620804
8. Identification and characterization of a family of mammalian methyl-CpG binding proteins.  |  Hendrich, B. and Bird, A. 1998. Mol Cell Biol. 18: 6538-47. PMID: 9774669

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBD2 Antibody (C-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MBD2 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBD2 Antibody (C-11) Specifications

Alternate Product Name: Methyl-CpG Binding Domain Protein 2

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-70

Epitope Target: MBD2

Epitope Species: human

Product Reactivity/Detection: MBD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MBD2 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MBD2/3 antibody is MBD2/3 Antibody (D-7): sc-271562.