MBD2/3 Antibody (D-7): sc-271562

MBD2/3 Antibody (D-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects the MBD2/3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MBD2/3 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MBD2/3 plays a crucial role in the regulation of gene expression through involvement in DNA methylation, a key epigenetic modification that influences transcriptional activity. Specifically, MBD2 and MBD3 are part of the methyl-CpG binding domain protein family, which recognizes and binds to methylated cytosine residues in DNA, thereby mediating transcriptional repression. This interaction is vital for maintaining cellular identity and function, as aberrant methylation patterns can lead to various diseases, including cancer. MBD2 associates with histone deacetylase 1 (HDAC1) within the MeCP1 repressor complex, further enhancing transcriptional silencing capabilities. Expression of MBD proteins is predominantly observed in somatic tissues, highlighting their importance in the regulation of gene expression across different cell types. Using anti-MBD2/3 antibody (D-7) in research can provide insights into the mechanisms of gene regulation and the implications of DNA methylation in health and disease.

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MBD2/3 Antibody (D-7) References:

1. Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development.  |  Hendrich, B., et al. 2001. Genes Dev. 15: 710-23. PMID: 11274056
2. Role of MBD2 in gene regulation and tumorigenesis.  |  Berger, J. and Bird, A. 2005. Biochem Soc Trans. 33: 1537-40. PMID: 16246164
3. Mbd3/NURD complex regulates expression of 5-hydroxymethylcytosine marked genes in embryonic stem cells.  |  Yildirim, O., et al. 2011. Cell. 147: 1498-510. PMID: 22196727
4. Epigenetic memory, MBD2 and the endothelium.  |  Zaina, S. and Lund, G. 2012. Curr Opin Lipidol. 23: 78-9. PMID: 22241329
5. Emerging Molecular and Biological Functions of MBD2, a Reader of DNA Methylation.  |  Wood, KH. and Zhou, Z. 2016. Front Genet. 7: 93. PMID: 27303433
6. A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to regulate transcription at active genes.  |  Zhang, T., et al. 2018. Nat Commun. 9: 3798. PMID: 30228260
7. Glioma stem-like cells evade interferon suppression through MBD3/NuRD complex-mediated STAT1 downregulation.  |  Zhan, X., et al. 2020. J Exp Med. 217: PMID: 32181805
8. MBD3 promotes hepatocellular carcinoma progression and metastasis through negative regulation of tumour suppressor TFPI2.  |  Yan, W., et al. 2022. Br J Cancer. 127: 612-623. PMID: 35501390
9. MBD3 as a Potential Biomarker for Colon Cancer: Implications for Epithelial-Mesenchymal Transition (EMT) Pathways.  |  Ding, Y., et al. 2023. Cancers (Basel). 15: PMID: 37370795
10. Analysis of the complex between MBD2 and the histone deacetylase core of NuRD reveals key interactions critical for gene silencing.  |  Leighton, GO., et al. 2023. Proc Natl Acad Sci U S A. 120: e2307287120. PMID: 37552759

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBD2/3 Antibody (D-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MBD2/3 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBD2/3 Antibody (D-7) Specifications

Alternate Product Name: Methyl-CpG-Binding Domain 2/3

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 242-291

Epitope Target: MBD2/3

Epitope Species: human

Product Reactivity/Detection: MBD2 and MBD3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MBD2/3 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MBD3 antibody is MBD3 Antibody (F-11): sc-166319.