Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAT Iα Double Nickase Plasmid (h): sc-405476-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAT Iα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAT Iα Double Nickase Plasmid (h) and MAT Iα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAT1A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAT Iα Double Nickase Plasmid (h) | sc-405476-NIC | 20 µg | $410.00 | |||
MAT Iα Double Nickase Plasmid (h2) | sc-405476-NIC-2 | 20 µg | $410.00 |
MAT1A encodes methionine adenosyltransferase I alpha (MAT Iα), a liver-enriched enzyme that catalyzes conversion of methionine and ATP to S-adenosylmethionine (SAM), the principal methyl donor for DNA, RNA, protein, and lipid methylation reactions. By governing intracellular SAM availability, MAT Iα links methionine and one-carbon metabolism to epigenetic regulation, redox homeostasis, and polyamine biosynthesis. Altered MAT1A expression or activity can perturb methylation potential and metabolic flux, processes frequently examined in studies of hepatic dysfunction, metabolic reprogramming, and methylation-associated phenotypes. As a result, MAT1A is widely used to interrogate connections between nutrient sensing, chromatin state, and downstream transcriptional programs in human cells.
MAT Iα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAT1A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAT1A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAT1A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAT1A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.