Mast Cell Chymase Antibody (CC1): sc-59586
- Mast Cell Chymase Antibody (CC1) is a mouse monoclonal IgG1 κ Mast Cell Chymase antibody, cited in 17 publications, provided at 200 µg/ml
- raised against purified skin chymase of human origin
- Mast Cell Chymase Antibody (CC1) is recommended for detection of Mast Cell Chymase distributed in skin, synovium, lung and heart of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including and porcine and canine
- Anti-Mast Cell Chymase Antibody (CC1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Mast Cell Chymase Antibody (CC1). This work is in progress.
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Mast Cell Chymase Antibody (CC1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mast Cell Chymase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Mast Cell Chymase (CC1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mast Cell Chymase, also known as CMA1 or MCT1, plays a crucial role in the immune response by generating vasoactive peptides and degrading the extracellular matrix, which is essential for tissue remodeling and inflammation. Mast Cell Chymase is primarily located in mast cells, which are specialized immune cells that release mediators during allergic reactions and inflammatory processes. The presence of Mast Cell Chymase in these cells highlights its importance in regulating local immune responses and maintaining tissue homeostasis. The human chymase gene, located on chromosome 14q11.2, encodes a preproenzyme that includes a 19 amino acid signal peptide, an acidic 2 amino acid propeptide, and a 226 amino acid catalytic domain, emphasizing its complex structure and functional significance. Mast Cell Chymase is classified as a chymotryptic serine proteinase and belongs to the peptidase family S1, which underscores its enzymatic activity in processing various substrates. Additionally, mouse and rat Mast Cell Protease 1 proteins, which are homologs to human tryptases, share 76% sequence identity at the amino acid level, making them valuable for studying mast cell biology and their roles in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Mast Cell Chymase Antibody (CC1) References:
- The diverse roles of mast cells. | Gurish, MF. and Austen, KF. 2001. J Exp Med. 194: F1-5. PMID: 11435478
- Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases. | Huang, RY., et al. 1991. Eur J Immunol. 21: 1611-21. PMID: 2060576
- Structure, chromosomal assignment, and deduced amino acid sequence of a human gene for mast cell chymase. | Caughey, GH., et al. 1991. J Biol Chem. 266: 12956-63. PMID: 2071582
- The human mast cell chymase gene (CMA1): mapping to the cathepsin G/granzyme gene cluster and lineage-restricted expression. | Caughey, GH., et al. 1993. Genomics. 15: 614-20. PMID: 8468056
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mast Cell Chymase Antibody (CC1) | sc-59586 | 200 µg/ml | $322.00 | |||
Mast Cell Chymase Antibody (CC1) AC | sc-59586 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Mast Cell Chymase Antibody (CC1) HRP | sc-59586 HRP | 200 µg/ml | $322.00 | |||
Mast Cell Chymase Antibody (CC1) FITC | sc-59586 FITC | 200 µg/ml | $336.00 | |||
Mast Cell Chymase Antibody (CC1) PE | sc-59586 PE | 200 µg/ml | $349.00 | |||
Mast Cell Chymase Antibody (CC1) Alexa Fluor® 488 | sc-59586 AF488 | 200 µg/ml | $364.00 | |||
Mast Cell Chymase Antibody (CC1) Alexa Fluor® 546 | sc-59586 AF546 | 200 µg/ml | $364.00 | |||
Mast Cell Chymase Antibody (CC1) Alexa Fluor® 594 | sc-59586 AF594 | 200 µg/ml | $364.00 | |||
Mast Cell Chymase Antibody (CC1) Alexa Fluor® 647 | sc-59586 AF647 | 200 µg/ml | $364.00 | |||
Mast Cell Chymase Antibody (CC1) Alexa Fluor® 680 | sc-59586 AF680 | 200 µg/ml | $364.00 | |||
Mast Cell Chymase Antibody (CC1) Alexa Fluor® 790 | sc-59586 AF790 | 200 µg/ml | $364.00 |