Mast cell Antibody (MCG35): sc-53365
- Mast cell Antibody (MCG35) is a mouse monoclonal IgG1 κ Mast cell antibody provided at 200 µg/ml
- raised against spleen cells and bone marrow cells of human origin
- Mast cell Antibody (MCG35) is recommended for detection of Mast cell of human origin by IF
- Anti-Mast cell Antibody (MCG35) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Mast cell Antibody (MCG35). These reagents are now offered in bundles with Mast cell Antibody (MCG35) (see ordering information below).
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Mast cell Antibody (MCG35) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mast cell protein of human origin by immunofluorescence (IF). Mast cell (MCG35) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Mast cells, or mastocytes, are primarily located in connective tissues near blood vessels and mucosal membranes, where they play crucial roles in immune responses. These cells are rich in histamine and heparin, which are vital for their function in allergic reactions and defense against pathogens. Upon activation, mast cells release granules containing these mediators, leading to rapid physiological responses such as vasodilation and increased vascular permeability. This process is essential not only for the development of allergies and anaphylaxis but also for wound healing and pathogen defense. Furthermore, mast cells are implicated in various autoimmune disorders, including rheumatoid arthritis and multiple sclerosis, highlighting their importance in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Mast cell Antibody (MCG35) References:
- Mast cells, basophils, and eosinophils: distinct but overlapping pathways for recruitment. | Bochner, BS. and Schleimer, RP. 2001. Immunol Rev. 179: 5-15. PMID: 11292027
- 5. IgE, mast cells, basophils, and eosinophils. | Prussin, C. and Metcalfe, DD. 2006. J Allergy Clin Immunol. 117: S450-6. PMID: 16455345
- The role of mast cells and eosinophils in chronic gastritis. | Moorchung, N., et al. 2006. Clin Exp Med. 6: 107-14. PMID: 17061058
- [Mast cells and basophilic granulocytes: their significance in allergic diseases]. | Bruijnzeel, PL., et al. 1991. Schweiz Med Wochenschr. 121: 1675-85. PMID: 1957141
- Role of human basophils and mast cells in the pathogenesis of allergic diseases. | Schleimer, RP., et al. 1985. J Allergy Clin Immunol. 76: 369-74. PMID: 2410478
- Origin of human mast cells studied by dual immunofluorescence. | Rimmer, EF. and Horton, MA. 1987. Clin Exp Immunol. 68: 712-8. PMID: 3308221
- Human mast cells detected by monoclonal antibodies. | Rimmer, EF., et al. 1984. J Clin Pathol. 37: 1249-55. PMID: 6389604
- [Effector cells in allergy: biological principles and new pharmacologic concepts]. | Sperr, WR., et al. 1993. Wien Klin Wochenschr. 105: 677-81. PMID: 7508662
- The cells of the allergic response: mast cells, basophils, and eosinophils. | Costa, JJ., et al. 1997. JAMA. 278: 1815-22. PMID: 9396642
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mast cell Antibody (MCG35) | sc-53365 | 200 µg/ml | $322.00 | |||
Mast cell Antibody (MCG35): m-IgG Fc BP-HRP Bundle | sc-526801 | 200 µg Ab; 10 µg BP | $361.00 | |||
Mast cell Antibody (MCG35): m-IgG1 BP-HRP Bundle | sc-532174 | 200 µg Ab; 20 µg BP | $361.00 | |||
Mast Cell Antibody (MCG35) AC | sc-53365 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Mast Cell Antibody (MCG35) HRP | sc-53365 HRP | 200 µg/ml | $322.00 | |||
Mast Cell Antibody (MCG35) FITC | sc-53365 FITC | 200 µg/ml | $336.00 | |||
Mast Cell Antibody (MCG35) PE | sc-53365 PE | 200 µg/ml | $349.00 | |||
Mast Cell Antibody (MCG35) Alexa Fluor® 488 | sc-53365 AF488 | 200 µg/ml | $364.00 | |||
Mast cell Antibody (MCG35) Alexa Fluor® 546 | sc-53365 AF546 | 200 µg/ml | $364.00 | |||
Mast Cell Antibody (MCG35) Alexa Fluor® 594 | sc-53365 AF594 | 200 µg/ml | $364.00 | |||
Mast Cell Antibody (MCG35) Alexa Fluor® 647 | sc-53365 AF647 | 200 µg/ml | $364.00 | |||
Mast Cell Antibody (MCG35) Alexa Fluor® 680 | sc-53365 AF680 | 200 µg/ml | $364.00 | |||
Mast Cell Antibody (MCG35) Alexa Fluor® 790 | sc-53365 AF790 | 200 µg/ml | $364.00 |