Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Maspin Double Nickase Plasmid (h): sc-416693-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Maspin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Maspin Double Nickase Plasmid (h) and Maspin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SERPINB5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Maspin Antibody (C-8): sc-271694
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Maspin Double Nickase Plasmid (h) | sc-416693-NIC | 20 µg | $410.00 | |||
Maspin Double Nickase Plasmid (h2) | sc-416693-NIC-2 | 20 µg | $410.00 |
SERPINB5 encodes Maspin, a non-classical serine protease inhibitor family member that functions as a context-dependent regulator of epithelial cell adhesion, motility, and extracellular matrix interactions. Maspin influences cytoskeletal remodeling and cell–matrix signaling programs linked to migration and invasion, and has been reported to modulate responses to oxidative stress and chromatin-associated regulation through interactions with nuclear and cytoplasmic partners. Altered SERPINB5 expression and subcellular localization have been associated with tumor progression phenotypes in multiple tissue types, making it a useful molecular node for studying mechanisms of metastasis-related cell behavior. As a biomarker-linked gene in cancer biology, SERPINB5 is frequently interrogated in models of epithelial differentiation, invasion, and microenvironmental remodeling.
Maspin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SERPINB5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SERPINB5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SERPINB5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SERPINB5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.